Evidence map›Paper›PMID 28835665›Full record

ArticleScientific reports2017

Optical Control of Tumor Induction in the Zebrafish.

Zhiping Feng, Suzy Nam, Fatima Hamouri, Isabelle Aujard, Bertrand Ducos, Sophie Vriz, Michel Volovitch, Ludovic Jullien, Shuo Lin, Shimon Weiss and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Zebrafish Models of Paediatric Brain Tumours.International journal of molecular sciences · 2022
    Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Cell-Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code.Chembiochem : a European journal of chemical biology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 3 countries.

Zhiping FengDepartment of Molecular, Cellular and Integrative Physiology, University of California at Los Angeles, Los Angeles, California, USA. zpfeng@stanford.edu.
Suzy NamDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, California, USA.
Fatima HamouriLaboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Paris, France.
Isabelle AujardÉcole Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, Paris, France.
Bertrand DucosLaboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Paris, France.
Sophie VrizCenter for Interdisciplinary Research in Biology (CIRB), College de France, and CNRS UMR 7241, and INSERM U1050, Paris, France.
Michel VolovitchCenter for Interdisciplinary Research in Biology (CIRB), College de France, and CNRS UMR 7241, and INSERM U1050, Paris, France.
Ludovic JullienÉcole Normale Supérieure, PSL Research University, UPMC Univ Paris 06, CNRS, Département de Chimie, PASTEUR, Paris, France.
Shuo LinDepartment of Molecular, Cell and Developmental Biology, University of California at Los Angeles, Los Angeles, California, USA.
Shimon WeissDepartment of Molecular, Cellular and Integrative Physiology, University of California at Los Angeles, Los Angeles, California, USA. sweiss@chem.ucla.edu.
David BensimonLaboratoire de Physique Statistique, Ecole Normale Supérieure, PSL Research University, Paris, France. david@lps.ens.fr.
University of California, Los Angeles · USCentre National de la Recherche Scientifique · FRÉcole Normale Supérieure - PSL · FRInserm · FRInstitut de Biologie de l'École Normale Supérieure · FRSorbonne Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The zebrafish has become an increasingly popular and valuable cancer model over the past few decades. While most zebrafish cancer models are generated by expressing mammalian oncogenes under tissue-specific promoters, here we describe a method that allows for the precise optical control of oncogene expression in live zebrafish. We utilize this technique to transiently or constitutively activate a typical human oncogene, kRASG12V, in zebrafish embryos and investigate the developmental and tumorigenic phenotypes. We demonstrate the spatiotemporal control of oncogene expression in live zebrafish, and characterize the different tumorigenic probabilities when kRASG12V is expressed transiently or constitutively at different developmental stages. Moreover, we show that light can be used to activate oncogene expression in selected tissues and single cells without tissue-specific promoters. Our work presents a novel approach to initiate and study cancer in zebrafish, and the high spatiotemporal resolution of this method makes it a valuable tool for studying cancer initiation from single cells.

Indexed as

Cell Transformation, NeoplasticAnimalsBiomarkers, TumorDisease Models, AnimalGene Expression Regulation, NeoplasticHumansMutationNeoplasmsOncogenesProto-Oncogene Proteins p21(ras)Transcriptional ActivationZebrafishBiomarkers, TumorKRAS protein, humanProto-Oncogene Proteins p21(ras)

Identifiers

PMID28835665
PMCPMC5569104
OpenAlexW2750133429

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.