ArticleScientific reports2017
BioFlow: a non-invasive, image-based method to measure speed, pressure and forces inside living cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- OpticalFlow3D - a tool for measuring amorphous motion in three-dimensional fluorescence microscopy images.Journal of cell science · 2026Article
- Fibronectin induces a transition from amoeboid to a fan morphology and modifies migration in Entamoeba histolytica.PLoS pathogens · 2024Article
- u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions.Cell reports methods · 2023Article
- 4D live imaging and computational modeling of a functional gut-on-a-chip evaluate how peristalsis facilitates enteric pathogen invasion.Science advances · 2022Article
- Chemokine-biased robust self-organizing polarization of migrating cells in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow.Nature communications · 2020Article
- Optical flow analysis reveals that Kinesin-mediated advection impacts the orientation of microtubules in theMolecular biology of the cell · 2020Article
- 3D flow field estimation and assessment for live cell fluorescence microscopy.Bioinformatics (Oxford, England) · 2020Article
- Article
- Cytoplasmic flows in starfish oocytes are fully determined by cortical contractions.PLoS computational biology · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Cell motility is governed by a complex molecular machinery that converts physico-chemical cues into whole-cell movement. Understanding the underlying biophysical mechanisms requires the ability to measure physical quantities inside the cell in a simple, reproducible and preferably non-invasive manner. To this end, we developed BioFlow, a computational mechano-imaging method and associated software able to extract intracellular measurements including pressure, forces and velocity everywhere inside freely moving cells in two and three dimensions with high spatial resolution in a non-invasive manner. This is achieved by extracting the motion of intracellular material observed using fluorescence microscopy, while simultaneously inferring the parameters of a given theoretical model of the cell interior. We illustrate the power of BioFlow in the context of amoeboid cell migration, by modelling the intracellular actin bulk flow of the parasite Entamoeba histolytica using fluid dynamics, and report unique experimental measures that complement and extend both theoretical estimations and invasive experimental measures. Thanks to its flexibility, BioFlow is easily adaptable to other theoretical models of the cell, and alleviates the need for complex or invasive experimental conditions, thus constituting a powerful tool-kit for mechano-biology studies. BioFlow is open-source and freely available via the Icy software.
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