Evidence map›Paper›PMID 28833845›Full record

ArticleJournal of applied microbiology2017

Detection of Bacillus anthracis spores from environmental water using bioluminescent reporter phage.

C Nguyen, R Makkar, N J Sharp, M A Page, I J Molineux, D A Schofield

Open access · bronzeAbstract readEvaluation Study
In one paragraph

Article in Journal of applied microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

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  7. Proceedings of the 7th ACM International Conference on Nanoscale Computing and Communication : Virtual Conference, September 23-25, 2020 : NanoCom 2020. ACM International Conference on Nanoscale Computing and Communication (7th : 2020 :... · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

C NguyenGuild BioSciences, Charleston, SC, USA.
R MakkarGuild BioSciences, Charleston, SC, USA.
N J SharpGuild BioSciences, Charleston, SC, USA.
M A PageConstruction Engineering and Research Laboratory, U.S. Army Corps of Engineers, Engineer Research and Development Center, Champaign, IL, USA.
I J MolineuxDepartment of Molecular BioSciences, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
D A SchofieldGuild BioSciences, Charleston, SC, USA.
The University of Texas at Austin · USUnited States Army Corps of Engineers · US

Funding

Detection of Biodefense Pathogens Using Phage DiagnosticsR01AI111535 · NIAID · GUILD ASSOCIATES, INC. · PI SCHOFIELD, DAVID A · 2014 to 2016
$1.6M
NIAID NIH HHS R01 AI111535
6 · The paper itself

Abstract

aimsWe investigated the ability of a temperate Bacillus anthracis reporter phage (Wβ::luxAB-2), which transduces bioluminescence to infected cells, to detect viable spores from deliberately contaminated environmental water samples. METHODS AND

resultsEnvironmental water was inoculated with spores and assayed with Wβ::luxAB-2. Bioluminescent signals directly correlated with input phage and spore concentrations. A limit of detection of 10

conclusionsThe reporter phage Wβ::luxAB-2 displays potential for simplified detection of viable spores from contaminated water samples within 12 h. SIGNIFICANCE AND IMPACT OF THE STUDY: A deliberate aerosol release of spores could lead to widespread contamination, leaving large areas uninhabitable until remediation. An essential requirement of this restoration process is the development of simplified detection assays in different environmental matrices.

Indexed as

Bacillus anthracisBacteriophagesBiosensing TechniquesGenes, ReporterLakesLuminescent MeasurementsPondsSpores, BacterialWater PollutionBacillusbioluminescencedetectionphagespores

Identifiers

PMID28833845
PMCPMC5650510
OpenAlexW2750042862

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.