ArticlePloS one2017
Serological and molecular tools to diagnose visceral leishmaniasis: 2-years' experience of a single center in Northern Italy.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Revisiting visceral leishmaniasis in immunocompromised patients: Ongoing gaps and advances in diagnostics, therapies, and preventive measures.Current research in parasitology & vector-borne diseases · 2026Review
- First documented case of visceral leishmaniasis with rare colonic involvement in an immunocompetent adult in Slovakia.BMC infectious diseases · 2025Article
- Re-emergence of human leishmaniasis in northern Italy, 2004 to 2022: a retrospective analysis.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Observational
- A 10-Year Retrospective Study on Pediatric Visceral Leishmaniasis in a European Endemic Area: Diagnostic and Short-Course Therapeutic Strategies.Healthcare (Basel, Switzerland) · 2023Article
- The estimated distribution of autochthonous leishmaniasis by Leishmania infantum in Europe in 2005-2020.PLoS neglected tropical diseases · 2023Review
- Updated diagnosis and graft involvement for visceral leishmaniasis in kidney transplant recipients: a case report and literature review.Infection · 2023Review
- Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods.Parasites & vectors · 2022Review
- Visceral Leishmaniasis: Epidemiology, Diagnosis, and Treatment Regimens in Different Geographical Areas with a Focus on Pediatrics.Microorganisms · 2022Review
- ELISA-based evaluation of antibody response to Leishmania in a region endemic for cutaneous leishmaniasis.Parasite immunology · 2022Article
- Test combination to detect latent Leishmania infection: A prevalence study in a newly endemic area for L. infantum, northeastern Italy.PLoS neglected tropical diseases · 2022Article
- Leishmaniases in the European Union and Neighboring Countries.Emerging infectious diseases · 2021Article
- Identification ofParasitology · 2021Article
- Serodiagnosis of Visceral Leishmaniasis in Northeastern Italy: Evaluation of Seven Serological Tests.Microorganisms · 2020Article
- Place of Serology in the Diagnosis of Zoonotic Leishmaniases With a Focus on Visceral Leishmaniasis Due toFrontiers in cellular and infection microbiology · 2020Review
- Clinical aspects of visceral leishmaniasis caused by L. infantum in adults. Ten years of experience of the largest outbreak in Europe: what have we learned?Parasites & vectors · 2019Observational
- Two cases of relapsed HIV-associated visceral leishmaniasis successfully treated with combination therapy.AIDS research and therapy · 2018Article
- Validation of rK39 immunochromatographic test and direct agglutination test for the diagnosis of Mediterranean visceral leishmaniasis in Spain.PLoS neglected tropical diseases · 2018Article
Corrections and comments
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Authors and funding
19 authors at 7 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The diagnosis of visceral leishmaniasis (VL) remains challenging, due to the limited sensitivity of microscopy, the poor performance of serological methods in immunocompromised patients and the lack of standardization of molecular tests. The aim of this study was to implement a combined diagnostic workflow by integrating serological and molecular tests with standardized clinical criteria. Between July 2013 and June 2015, the proposed workflow was applied to specimens obtained from 94 in-patients with clinical suspicion of VL in the Emilia-Romagna region, Northern Italy. Serological tests and molecular techniques were employed. Twenty-one adult patients (22%) had a confirmed diagnosis of VL by clinical criteria, serology and/or real-time polymerase chain reaction; 4 of these patients were HIV-positive. Molecular tests exhibited higher sensitivity than serological tests for the diagnosis of VL. In our experience, the rK39 immunochromatographic test was insufficiently sensitive for use as a screening test for the diagnosis of VL caused by L. infantum in Italy. However, as molecular tests are yet not standardized, further studies are required to identify an optimal screening test for Mediterranean VL.
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