ArticlePloS one2017
Analysis of repetitive amino acid motifs reveals the essential features of spider dragline silk proteins.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 84 citations in OpenAlex.
- Design and Heterologous Production of Bagworm Silk-Inspired Proteins inComputational and structural biotechnology journal · 2026Article
- Spider silk-based structural proteins as tough, biodegradable, and sustainable polymers.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2026Review
- Nanostructured Protein Surfaces Inspired by Spider Silk.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Recombinant production of spider silk protein in Physcomitrella photobioreactors.Plant cell reports · 2025Article
- Native Silk Fibers: Protein Sequence and Structure Influences on Thermal and Mechanical Properties.Biomacromolecules · 2025Review
- The Biology of Natural Polymers Accelerates and Expands the Science of Biomacromolecules: A Focus on Structural Proteins.Biomacromolecules · 2025Review
- Strategies for Making High-Performance Artificial Spider Silk Fibers.Advanced functional materials · 2024Review
- Glycine rich proteins of ticks: more than a cement component.Parasitology · 2024Review
- The role of flow in the self-assembly of dragline spider silk proteins.Biophysical journal · 2023Article
- Novel insights into construct toxicity, strain optimization, and primary sequence design for producing recombinant silk fibroin and elastin-like peptide inMetabolic engineering communications · 2023Article
- Relating spidroin motif prevalence and periodicity to the mechanical properties of major ampullate spider silks.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2023Article
- Chromosome-level genome and the identification of sex chromosomes in Uloborus diversus.GigaScience · 2022Article
- Rapid molecular diversification and homogenization of clustered major ampullate silk genes in Argiope garden spiders.PLoS genetics · 2022Article
- Review
- 1000 spider silkomes: Linking sequences to silk physical properties.Science advances · 2022Article
- Protein secondary structure in spider silk nanofibrils.Nature communications · 2022Article
- Complexity of Spider Dragline Silk.Biomacromolecules · 2022Review
- Mantle Transcriptome Provides Insights into Biomineralization and Growth Regulation in the Eastern Oyster (Crassostrea virginica).Marine biotechnology (New York, N.Y.) · 2022Article
- Darwin's bark spider shares a spidroin repertoire withOpen biology · 2021Article
- Temperature-dependent modulation by biaryl-based monomers of the chain length and morphology of biphenyl-based supramolecular polymers.Chemical science · 2021Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extraordinary mechanical properties of spider dragline silk are dependent on the highly repetitive sequences of the component proteins, major ampullate spidroin 1 and 2 (MaSp2 and MaSp2). MaSp sequences are dominated by repetitive modules composed of short amino acid motifs; however, the patterns of motif conservation through evolution and their relevance to silk characteristics are not well understood. We performed a systematic analysis of MaSp sequences encompassing infraorder Araneomorphae based on the conservation of explicitly defined motifs, with the aim of elucidating the essential elements of MaSp1 and MaSp2. The results show that the GGY motif is nearly ubiquitous in the two types of MaSp, while MaSp2 is invariably associated with GP and di-glutamine (QQ) motifs. Further analysis revealed an extended MaSp2 consensus sequence in family Araneidae, with implications for the classification of the archetypal spidroins ADF3 and ADF4. Additionally, the analysis of RNA-seq data showed the expression of a set of distinct MaSp-like variants in genus Tetragnatha. Finally, an apparent association was uncovered between web architecture and the abundance of GP, QQ, and GGY motifs in MaSp2, which suggests a co-expansion of these motifs in response to the evolution of spiders' prey capture strategy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.