ArticleBiochemistry2017
The Conformational Flexibility of the Acyltransferase from the Disorazole Polyketide Synthase Is Revealed by an X-ray Free-Electron Laser Using a Room-Temperature Sample Delivery Method for Serial Crystallography.
Article in Biochemistry, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- A simple and practical approach for protein serial crystallography using grease matrix and large-area support film.Journal of synchrotron radiation · 2026Article
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Article
- Structure and Mechanisms of Assembly-Line Polyketide Synthases.Annual review of biochemistry · 2024Review
- Engineering site-selective incorporation of fluorine into polyketides.Nature chemical biology · 2022Article
- Polysaccharide-Based Injection Matrix for Serial Crystallography.International journal of molecular sciences · 2020Article
- Advances in methods for atomic resolution macromolecular structure determination.F1000Research · 2020Review
- 3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating.Journal of applied crystallography · 2019Article
- An outlook on using serial femtosecond crystallography in drug discovery.Expert opinion on drug discovery · 2019Review
- Nylon mesh-based sample holder for fixed-target serial femtosecond crystallography.Scientific reports · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Acyltransferases as Tools for Polyketide Synthase Engineering.Antibiotics (Basel, Switzerland) · 2018Review
- Microfocus diffraction from different regions of a protein crystal: structural variations and unit-cell polymorphism.Acta crystallographica. Section D, Structural biology · 2018Article
Corrections and comments
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
Abstract
The crystal structure of the trans-acyltransferase (AT) from the disorazole polyketide synthase (PKS) was determined at room temperature to a resolution of 2.5 Å using a new method for the direct delivery of the sample into an X-ray free-electron laser. A novel sample extractor efficiently delivered limited quantities of microcrystals directly from the native crystallization solution into the X-ray beam at room temperature. The AT structure revealed important catalytic features of this core PKS enzyme, including the occurrence of conformational changes around the active site. The implications of these conformational changes for polyketide synthase reaction dynamics are discussed.
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Registered trials
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