ArticleAMB Express2017
Survey of methanotrophic diversity in various ecosystems by degenerate methane monooxygenase gene primers.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
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- Leafcutter ants enhance microbial drought resilience in tropical forest soil.Environmental microbiology reports · 2024Article
- Acidophilic methanotrophs: Occurrence, diversity, and possible bioremediation applications.Environmental microbiology reports · 2023Review
- Prospecting the significance of methane-utilizing bacteria in agriculture.World journal of microbiology & biotechnology · 2022Review
- Universal activity-based labeling method for ammonia- and alkane-oxidizing bacteria.The ISME journal · 2022Article
- Methylotrophs and Hydrocarbon-Degrading Bacteria Are Key Players in the Microbial Community of an Abandoned Century-Old Oil Exploration Well.Microbial ecology · 2022Article
- Warming and eutrophication interactively drive changes in the methane-oxidizing community of shallow lakes.ISME communications · 2021Article
- Methane utilizing plant growth-promoting microbial diversity analysis of flooded paddy ecosystem of India.World journal of microbiology & biotechnology · 2021Article
- Novel facultative Methylocella strains are active methane consumers at terrestrial natural gas seeps.Microbiome · 2019Article
- Article
- Article
- Complete Genome Sequence of the Aerobic Facultative Methanotroph Methylocella tundrae Strain T4.Microbiology resource announcements · 2019Article
- Activity and Identification of Methanotrophic Bacteria in Arable and No-Tillage Soils from Lublin Region (Poland).Microbial ecology · 2019Article
- Article
- Unravelling the Identity, Metabolic Potential and Global Biogeography of the Atmospheric Methane-Oxidizing Upland Soil Cluster α.Environmental microbiology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Methane is the second most important greenhouse gas contributing to about 20% of global warming. Its mitigation is conducted by methane oxidizing bacteria that act as a biofilter using methane as their energy and carbon source. Since their first discovery in 1906, methanotrophs have been studied using a complementary array of methods. One of the most used molecular methods involves PCR amplification of the functional gene marker for the diagnostic of copper and iron containing particulate methane monooxygenase. To investigate the diversity of methanotrophs and to extend their possible molecular detection, we designed a new set of degenerate methane monooxygenase primers to target an 850 nucleotide long sequence stretch from pmoC to pmoA. The primers were based on all available full genomic pmoCAB operons. The newly designed primers were tested on various pure cultures, enrichment cultures and environmental samples using PCR. The results demonstrated that this primer set has the ability to correctly amplify the about 850 nucleotide long pmoCA product from Alphaproteobacteria, Gammaproteobacteria, Verrucomicrobia and the NC10 phyla methanotrophs. The new primer set will thus be a valuable tool to screen ecosystems and can be applied in conjunction with previously used pmoA primers to extend the diversity of currently known methane-oxidizing bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.