ArticleScientific reports2017
Identification of candidate genes involved in isoquinoline alkaloids biosynthesis in Dactylicapnos scandens by transcriptome analysis.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 33 citations in OpenAlex.
- Integrated Metabolomics and Transcriptomics Analysis Reveals the Biosynthetic Mechanism of Isoquinoline Alkaloids in Different Tissues ofCurrent issues in molecular biology · 2026Article
- Biosynthesis of benzylisoquinoline alkaloids and its evolution in plants.Plant communications · 2026Review
- Iterative enrichment cultivation and multiomic analysis reveal potential endophytic bacteria affecting the sinomenine synthesis in Sinomenium acutum.Microbial cell factories · 2026Article
- Metabolomic Insights into Sexual Multi-Morphism of Sinomenine Accumulation inPlants (Basel, Switzerland) · 2025Article
- Review
- Cloning, heterologous expression, and expression analysis of SinSyn7 gene from Sinomenium acutum.PloS one · 2025Article
- Article
- Herbgenomics meets Papaveraceae: a promising -omics perspective on medicinal plant research.Briefings in functional genomics · 2024Review
- Ethnomedicine, phytochemistry, and pharmacological activities of Uvaria chamae P. Beauv.: A comprehensive review.Naunyn-Schmiedeberg's archives of pharmacology · 2024Review
- Article
- Transcriptome-Wide Characterization of Alkaloids and Chlorophyll Biosynthesis in Lotus Plumule.Frontiers in plant science · 2022Article
- The chromosome-level reference genome of Coptis chinensis provides insights into genomic evolution and berberine biosynthesis.Horticulture research · 2021Article
- Full-length transcriptome analysis of Coptis deltoidea and identification of putative genes involved in benzylisoquinoline alkaloids biosynthesis based on combined sequencing platforms.Plant molecular biology · 2020Article
- Isolation and characterization of twoThe Journal of biological chemistry · 2020Article
- Article
- Molecular Origins of Functional Diversity in Benzylisoquinoline Alkaloid Methyltransferases.Frontiers in plant science · 2019Review
- Rifamycin congeners kanglemycins are active against rifampicin-resistant bacteria via a distinct mechanism.Nature communications · 2018Article
- Identification and Characterization of Genes Involved in Benzylisoquinoline Alkaloid Biosynthesis inFrontiers in plant science · 2018Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Dactylicapnos scandens (D. Don) Hutch (Papaveraceae) is a well-known traditional Chinese herb used for treatment of hypertension, inflammation, bleeding and pain for centuries. Although the major bioactive components in this herb are considered as isoquinoline alkaloids (IQAs), little is known about molecular basis of their biosynthesis. Here, we carried out transcriptomic analysis of roots, leaves and stems of D. scandens, and obtained a total of 96,741 unigenes. Based on gene expression and phylogenetic relationship, we proposed the biosynthetic pathways of isocorydine, corydine, glaucine and sinomenine, and identified 67 unigenes encoding enzymes potentially involved in biosynthesis of IQAs in D. scandens. High performance liquid chromatography analysis demonstrated that while isocorydine is the most abundant IQA in D. scandens, the last O-methylation biosynthesis step remains unclear. Further enzyme activity assay, for the first time, characterized a gene encoding O- methyltransferase (DsOMT), which catalyzes O-methylation at C7 of (S)-corytuberine to form isocorydine. We also identified candidate transcription factor genes belonging to WRKY and bHLH families that may be involved in the regulation of IQAs biosynthesis. Taken together, we first provided valuable genetic information for D. scandens, shedding light on candidate genes involved in IQA biosynthesis, which will be critical for further gene functional characterization.
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