ArticleNature communications2017
Flow-induced elongation of von Willebrand factor precedes tension-dependent activation.
Article in Nature communications, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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The trial behind it
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Who cites it
117 citing papers in PubMed, 203 citations in OpenAlex.
- Shear-Activated von Willebrand Factor Captures Extracellular Vesicles to Promote Platelet Activation and Metastasis.Journal of extracellular vesicles · 2026Article
- Flow molecular dynamics simulations reveal mechano-presentation of von Willebrand factor through glycan-modulated autoinhibitory modules.APL bioengineering · 2026Article
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- The Effect of von Willebrand Disease on Platelet Adhesion Dynamics: Correlating a Multiscale Platelet Model to In Vitro Results.IEEE transactions on bio-medical engineering · 2026Article
- Mechanobiology of cancer-associated thrombosis: from molecular mechanisms to therapeutic innovation.Journal of nanobiotechnology · 2026Review
- Multimodal characterization of flow-induced thrombus initiation and growth in extracorporeal membrane oxygenation.Scientific reports · 2026Article
- Polymer embedding of membrane lungs for histological investigations of intra-device clot formation.Frontiers in cardiovascular medicine · 2026Article
- Arterial human blood flow can be stopped by cables of VWF and platelets without coagulation.Blood advances · 2025Article
- Multidimensional optimization of stent design for endothelial shear stress regulation: Geometric structuring, surface functionalization strategies to mitigate thrombosis and restenosis.International journal of cardiology. Heart & vasculature · 2025Review
- Hydrodynamic-derived centrifugal blood pump design for stable-low-flow-rate rate performance: from surface to structure.Bioactive materials · 2025Article
- Reversible formation of von-Willebrand-factor-platelet aggregates in microvascular blood flow.PNAS nexus · 2025Article
- Bleeding Complications from Chest Tube Treatment in Patients on Extracorporeal Membrane Oxygenation Support.Interdisciplinary cardiovascular and thoracic surgery · 2025Observational
- Key residues regulating von Willebrand factor A1/A2 interactions: insights from molecular dynamics simulations and experimental validation.Research and practice in thrombosis and haemostasis · 2025Article
- Vorticity-Facilitated Platelet Aggregation: a High Expansion-Ratio Stenotic Microfluidic Platform Unravels the Role of Complex Flow Dynamics in Arterial Thrombosis.Advanced healthcare materials · 2025Article
- Platelet recruitment kinetics are impacted by von Willebrand factor quality in hemostatic adjuncts.Blood vessels, thrombosis & hemostasis · 2025Article
- Von Willebrand disease (VWD) and pregnancy: a comprehensive overview.Thrombosis journal · 2025Review
- Dynamics of a von Willebrand Factor A1 Autoinhibitory Module with O-Linked Glycans and Its Roles in Regulation of GPIbα Binding.The journal of physical chemistry. B · 2025Article
- Role of von Willebrand factor (VWF), platelets, and aberrant flow in the initiation of venous thrombosis.Science advances · 2025Article
- The epitope of the antibody used in the REAADS VWF activity assay is quaternary.Thrombosis journal · 2025Article
- GPVI-mediated thrombus stabilization of shear-induced platelet aggregates in a microfluidic stenosis.Biophysical journal · 2025Article
57 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Von Willebrand factor, an ultralarge concatemeric blood protein, must bind to platelet GPIbα during bleeding to mediate hemostasis, but not in the normal circulation to avoid thrombosis. Von Willebrand factor is proposed to be mechanically activated by flow, but the mechanism remains unclear. Using microfluidics with single-molecule imaging, we simultaneously monitored reversible Von Willebrand factor extension and binding to GPIbα under flow. We show that Von Willebrand factor is activated through a two-step conformational transition: first, elongation from compact to linear form, and subsequently, a tension-dependent local transition to a state with high affinity for GPIbα. High-affinity sites develop only in upstream regions of VWF where tension exceeds ~21 pN and depend upon electrostatic interactions. Re-compaction of Von Willebrand factor is accelerated by intramolecular interactions and increases GPIbα dissociation rate. This mechanism enables VWF to be locally activated by hydrodynamic force in hemorrhage and rapidly deactivated downstream, providing a paradigm for hierarchical mechano-regulation of receptor-ligand binding.Von Willebrand factor (VWF) is a blood protein involved in clotting and is proposed to be activated by flow, but the mechanism is unknown. Here the authors show that VWF is first converted from a compact to linear form by flow, and is subsequently activated to bind GPIbα in a tension-dependent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.