ArticleAnnals of clinical microbiology and antimicrobials2017
Extreme diversity and multiple SCCmec elements in coagulase-negative Staphylococcus found in the Clinic and Community in Beijing, China.
Article in Annals of clinical microbiology and antimicrobials, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Community carriage of methicillin-resistant staphylococci among migrant communities living in Klang Valley, Malaysia.IJID regions · 2026Article
- Early detection of nosocomial pathogens in air and surfaces using an innovative genetic approach for surveillance in healthcare settings.Antimicrobial resistance and infection control · 2026Article
- Increase in methicillin-resistant Staphylococcus spp. colonization among pregnant individuals during COVID-19 pandemic.Scientific reports · 2024Article
- The Home Environment Is a Reservoir for Methicillin-Resistant Coagulase-Negative Staphylococci and Mammaliicocci.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Resistome, mobilome, and virulome explored in clinical isolates derived from acne patients in Egypt: unveiling unique traits of an emerging coagulase-negativeFrontiers in cellular and infection microbiology · 2024Article
- Whole-Genome Investigation of Zoonotic Transmission of Livestock-Associated Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- Identification and characterization of SCCmec typing with psm-mec positivity in staphylococci from patients with coagulase-negative staphylococci peritoneal dialysis-related peritonitis.BMC microbiology · 2023Article
- Novel Organization of the Staphylococcal Cassette ChromosomeMicrobiology spectrum · 2022Article
- Methicillin Resistant Staphylococci Isolated from Goats and Their Farm Environments in Saudi Arabia Genotypically Linked to Known Human Clinical Isolates: a Pilot Study.Microbiology spectrum · 2022Article
- Characterizing a Novel Staphylococcal Cassette ChromosomeAntimicrobial agents and chemotherapy · 2021Article
- Multidrug-resistant Staphylococcus cohnii and Staphylococcus urealyticus isolates from German dairy farms exhibit resistance to beta-lactam antibiotics and divergent penicillin-binding proteins.Scientific reports · 2021Article
- Improved Detection ofFrontiers in microbiology · 2021Article
- Distribution of Staphylococcal cassette chromosome mecA (SCCmec) types among coagulase-negativeIranian journal of basic medical sciences · 2020Article
- Emergence of linezolid-resistant Staphylococcus epidermidis in the tertiary children's hospital in Cracow, Poland.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Prevalence and antibiogram of coagulase negative Staphylococci in bioaerosols from different indoors of a university in India.BMC microbiology · 2020Article
- Whole genome sequencing revealed new molecular characteristics in multidrug resistant staphylococci recovered from high frequency touched surfaces in London.Scientific reports · 2019Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCoagulase-negative staphylococci (CoNS) are recognized as a large reservoir of staphylococcal cassette chromosome mec (SCCmec) harboured by Staphylococcus aureus. However, data of SCCmec in CoNS are relatively absent particularly in China.
methodsSeventy-eight CoNS clinical and 47 community isolates were collected in Beijing. PCR was performed to classify SCCmec types. Under oxacillin treatment, quantitative real-time reverse transcription PCR (qRT-PCR) was performed to compare mecA mRNA levels and mRNA half-life between isolates with single SCCmec element and those with multiple one. Their growth curves were analysed. Their bacterial cell wall integrity was also compared by performing a Gram stain. All ccr complex segments were sequenced and obtained ccr segments were analysed by phylogenetic analyses.
resultsAll 78 clinical isolates had mecA segments compared with 38% in community isolates (total 47). Only 29% clinical isolates and 33% community isolates (among mecA positive isolates) harboured a single previously identified SCCmec type; notably, 17% clinical isolates and 28% community isolates had multiple SCCmec types. Further studies indicated that isolates with multiple SCCmec elements had more stable mecA mRNA expression compared with isolates with single SCCmec elements. CoNS with multiple SCCmec elements demonstrated superior cell wall integrity. Interestingly, phylogenetic analyses of obtained 70 ccr segments indicated that horizontal gene transfer of the ccr complex might exist among various species of clinical CoNS, community CoNS and S. aureus.
conclusionsCoNS recovered from patients carried extremely diverse but distinctive SCCmec elements compared with isolates from the community. More attention should be given to CoNS with multiple SCCmec not only because they had superior cell wall integrity, but also because CoNS and S. aureus might acquire multiple SCCmec through the ccr complex.
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