ArticleBMC veterinary research2017
Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Stress, Well-Being, and Reproduction in Wildlife.Advances in experimental medicine and biology · 2026Review
- Feasibility and usefulness of in-hospital and at-home salivary sampling in healthy dogs and trilostane-treated dogs with Cushing's syndrome: a prospective observational study.BMC veterinary research · 2025Article
- Alpha-Amylase Activity in Feline Saliva: An Analytical Validation of an Automated Assay for Its Measurement and a Pilot Study on Its Changes Following Acute Stress and Due to Urinary Tract Pathologies.Animals : an open access journal from MDPI · 2025Article
- Welfare Assessment in Pigs Using the Salivary Proteome.Animals : an open access journal from MDPI · 2024Review
- Characteristics of the Digestive Tract of Dogs and Cats.Advances in experimental medicine and biology · 2024Article
- Investigating Dog Welfare When Interacting with Autistic Children within Canine-Assisted Occupational Therapy Sessions: A Single Case Study.Animals : an open access journal from MDPI · 2023Article
- From Chihuahua to Saint-Bernard: how did digestion and microbiota evolve with dog sizes.International journal of biological sciences · 2022Review
- The Markers of Stress in Swine Oral Fluid.Journal of veterinary research · 2021Article
- Salivary cortisol in healthy dogs: a randomized cross-over study to evaluate different saliva stimulation methods and their effects on saliva volume and cortisol concentration.BMC veterinary research · 2021Article
- Amino Acids in Dog Nutrition and Health.Advances in experimental medicine and biology · 2021Review
- Changes in Serum and Salivary Proteins in Canine Mammary Tumors.Animals : an open access journal from MDPI · 2020Article
- Changes in the Salivary Proteome Associated With Canine Pyometra.Frontiers in veterinary science · 2020Article
- Where Do We Stand in the Domestic Dog (Frontiers in psychology · 2020Review
- Variation of human salivary alpha-amylase proteoforms in three stimulation models.Clinical oral investigations · 2020Article
- Salivary alpha-amylase as a stress biomarker in diseased dogs.Journal of veterinary science · 2019Article
- Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study.Comparative immunology, microbiology and infectious diseases · 2018Article
- Changes in alpha-amylase activity, concentration and isoforms in pigs after an experimental acute stress model: an exploratory study.BMC veterinary research · 2018Article
- Salivary pH, calcium, phosphorus and selected enzymes in healthy dogs: a pilot study.BMC veterinary research · 2017Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSalivary alpha-amylase (sAA) is considered a biomarker of sympathetic activation in humans, but there is controversy regarding the existence of sAA in dogs. The hypothesis of this study was that sAA exists in dogs and it could change in situations of sympathetic stimulation. Therefore, the aims of this study were: 1) to demonstrate the presence of alpha-amylase in saliva of dogs by Western-Blot, 2) to validate an spectrophotometric method for the measurement of sAA activity and 3) to evaluate the possible changes in sAA activity after the induction of an ejaculation in dogs which is known to produce a sympathetic activation.
resultsWestern-Blot demonstrated a band in dog saliva specimens between 60 kDa and 50 kDa, similar to purified sAA. The spectrophotometric assay validated showed an adequate inter- and intra-assay precision, and a high correlation coefficient (r = 0.999) in the linearity under dilution study. sAA median activity significantly increased just after ejaculation compared with just before the ejaculation (2.06-fold, P = 0.005).
conclusionsThis study demonstrated the existence of alpha-amylase in saliva of dogs and that this enzyme can be measured by a spectrophotometric assay. In addition, results showed that sAA increase after a sympathetic activation and could be potentially used as non-invasive biomarker of sympathetic activity in this species.
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