Evidence map›Paper›PMID 28830447›Full record

ArticleJournal of nanobiotechnology2017

Nanostructured biosensor using bioluminescence quenching technique for glucose detection.

Longyan Chen, Longyi Chen, Michelle Dotzert, C W James Melling, Jin Zhang

Abstract read
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In one paragraph

Article in Journal of nanobiotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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  3. Research progress inExtracellular vesicles and circulating nucleic acids · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Longyan ChenDepartment of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON, N6A 5B9, Canada.
Longyi ChenDepartment of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON, N6A 5B9, Canada.
Michelle DotzertSchool of Kinesiology, Faculty of Health Sciences, University of Western Ontario, London, ON, N6A 5B9, Canada.
C W James MellingSchool of Kinesiology, Faculty of Health Sciences, University of Western Ontario, London, ON, N6A 5B9, Canada.
Jin ZhangDepartment of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, ON, N6A 5B9, Canada. jzhang@eng.uwo.ca.ORCID http://orcid.org/0000-0002-6875-8008

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMost methods for monitoring glucose level require an external energy source which may limit their application, particularly in vivo test. Bioluminescence technique offers an alternative way to provide emission light without external energy source by using bioluminescent proteins found from firefly or marine vertebrates and invertebrates. For quick and non-invasive detection of glucose, we herein developed a nanostructured biosensor by applying the bioluminescence technique.

resultsLuciferase bioluminescence protein (Rluc) is conjugated with β-cyclodextrin (β-CD). The bioluminescence intensity of Rluc can be quenched by 8 ± 3 nm gold nanoparticles (Au NPs) when Au NPs covalently bind to β-CD. In the presence of glucose, Au NPs are replaced and leave far from Rluc through a competitive reaction, which results in the restored bioluminescence intensity of Rluc. A linear relationship is observed between the restored bioluminescence intensity and the logarithmic glucose concentration in the range of 1-100 µM. In addition, the selectivity of this designed sensor has been evaluated. The performance of the senor for determination of the concentration of glucose in the blood of diabetic rats is studied for comparison with that of the concentration of glucose in aqueous.

conclusionsThis study demonstrates the design of a bioluminescence sensor for quickly detecting the concentration of glucose sensitively.

Indexed as

Animalsbeta-CyclodextrinsBioluminescence Resonance Energy Transfer TechniquesBiosensing TechniquesBlood GlucoseDiabetes Mellitus, ExperimentalGlucoseGoldHumansLuciferases, RenillaMaleMetal NanoparticlesRatsRats, Sprague-Dawleybeta-CyclodextrinsBlood GlucoseGlucoseGoldLuciferases, RenillaBioluminescenceBiosensorGlucoseNanoparticles

Identifiers

PMID28830447
PMCPMC5567885

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.