ArticleBMC microbiology2017
Genome sequencing and comparative genomics of enterohemorrhagic Escherichia coli O145:H25 and O145:H28 reveal distinct evolutionary paths and marked variations in traits associated with virulence & colonization.
Article in BMC microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Non-Melibiose Fermentation and Tellurite Resistance by Shigatoxigenic and EnteropathogenicVeterinary sciences · 2025Article
- Centriflaken: An automated data analysis pipeline for assembly and in silico analyses of foodborne pathogens from metagenomic samples.PloS one · 2025Article
- Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71.BMC veterinary research · 2024Article
- Increased resistance against tellurite is conferred by a mutation in the promoter region of uncommon tellurite resistance geneApplied and environmental microbiology · 2024Article
- Pathogenomes of Shiga Toxin Positive and NegativeMicroorganisms · 2024Article
- The enterohemorrhagic Escherichia coli insertion sequence-excision enhancer protein is a DNA polymerase with microhomology-mediated end-joining activity.Nucleic acids research · 2023Article
- The Prophage and Us-Shiga Toxin Phages Revisited.Pathogens (Basel, Switzerland) · 2023Article
- Article
- Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from theApplied and environmental microbiology · 2022Article
- Comparative Genomics Applied to Systematically Assess Pathogenicity Potential in Shiga Toxin-ProducingMicroorganisms · 2022Article
- The Importance of Shiga Toxin-ProducingMicroorganisms · 2022Article
- Prophages integrating into prophages: A mechanism to accumulate type III secretion effector genes and duplicate Shiga toxin-encoding prophages in Escherichia coli.PLoS pathogens · 2021Article
- Diversity of the Tellurite Resistance Gene Operon inFrontiers in microbiology · 2021Article
- Characterization of Shiga Toxin 2a Encoding Bacteriophages Isolated From High-Virulent O145:H25 Shiga Toxin-ProducingFrontiers in microbiology · 2021Article
- Article
- Is Shiga Toxin-ProducingMicroorganisms · 2020Article
- Standardized phylogenetic and molecular evolutionary analysis applied to species across the microbial tree of life.Scientific reports · 2020Article
- Genomic epidemiology and carbon metabolism of Escherichia coli serogroup O145 reflect contrasting phylogenies.PloS one · 2020Article
- Pathogenomes of Atypical Non-shigatoxigenicFrontiers in microbiology · 2020Article
- Comparative genomics reveals a lack of evidence for pigeons as a main source of stxBMC genomics · 2019Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnterohemorrhagic Escherichia coli (EHEC) O145 are among the top non-O157 serogroups associated with severe human disease worldwide. Two serotypes, O145:H25 and O145:H28 have been isolated from human patients but little information is available regarding the virulence repertoire, origin and evolutionary relatedness of O145:H25. Hence, we sequenced the complete genome of two O145:H25 strains associated with hemolytic uremic syndrome (HUS) and compared the genomes with those of previously sequenced O145:H28 and other EHEC strains.
resultsThe genomes of the two O145:H25 strains were 5.3 Mbp in size; slightly smaller than those of O145:H28 and other EHEC strains. Both strains contained three nearly identical plasmids and several prophages and integrative elements, many of which differed significantly in size, gene content and organization as compared to those present in O145:H28 and other EHECs. Furthermore, notable variations were observed in several fimbrial gene cluster and intimin types possessed by O145:H25 and O145:H28 indicating potential adaptation to distinct areas of host colonization. Comparative genomics further revealed that O145:H25 are genetically more similar to other non-O157 EHEC strains than to O145:H28.
conclusionPhylogenetic analysis accompanied by comparative genomics revealed that O145:H25 and O145:H28 evolved from two separate clonal lineages and that horizontal gene transfer and gene loss played a major role in the divergence of these EHEC serotypes. The data provide further evidence that ruminants might be a possible reservoir for O145:H25 but that they might be impaired in their ability to establish a persistent colonization as compared to other EHEC strains.
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