ArticleDevelopmental cell2017
A Single-Cell Biochemistry Approach Reveals PAR Complex Dynamics during Cell Polarization.
Article in Developmental cell, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed.
- Reciprocal E-cadherin signaling aligns apical surfaces between neighboring epithelial tissues to complete the C. elegans digestive tract.Current biology : CB · 2026Article
- Cell cycle oscillations in a polarity network facilitate state switching by morphogenetic cues.Science advances · 2026Article
- Asymmetric distribution of actin-related proteins in the earlybioRxiv : the preprint server for biology · 2026Article
- Cycloheximide-resistant ribosomes reveal adaptive translation dynamics in C. elegans.Genetics · 2026Article
- Intensity-Based Estimation of Monomeric Brightness for Fluorescent Proteins.International journal of molecular sciences · 2025Article
- Cell cycle oscillations in a polarity network facilitate state switching by morphogenetic cues.bioRxiv : the preprint server for biology · 2025Article
- Oligomerization and positive feedback on membrane recruitment encode dynamically stable PAR-3 asymmetries in thebioRxiv : the preprint server for biology · 2025Article
- Phosphorylation by Aurora kinase A facilitates cortical-cytoplasmic dynamics of Par-3 in asymmetric division of radial glia progenitors.Science advances · 2025Article
- Membrane extraction in native lipid nanodiscs reveals dynamic regulation of Cdc42 complexes during cell polarization.Biophysical journal · 2025Article
- Lipid Oxidation at the Crossroads: Oxidative Stress and Neurodegeneration Explored inAntioxidants (Basel, Switzerland) · 2025Review
- Internal feedback circuits among MEX-5, MEX-6, and PLK-1 maintain faithful patterning in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- A PDZ-kinase allosteric relay mediates Par complex regulator exchange.bioRxiv : the preprint server for biology · 2024Article
- Dimerization activates the Inversin complex inMolecular biology of the cell · 2024Article
- Optimized PAR-2 RING dimerization mediates cooperative and selective membrane binding for robust cell polarity.The EMBO journal · 2024Article
- PLK-1 regulates MEX-1 polarization in thebioRxiv : the preprint server for biology · 2024Article
- Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in thebioRxiv : the preprint server for biology · 2024Article
- A CDK1 phosphorylation site oneLife · 2024Article
- CaCommunications biology · 2024Article
- Cauchy hyper-graph Laplacian nonnegative matrix factorization for single-cell RNA-sequencing data analysis.BMC bioinformatics · 2024Article
- Temporally distinct roles of Aurora A in polarization of the C. elegans zygote.Development (Cambridge, England) · 2024Article
25 more citing papers are in PubMed but not listed here.
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5 authors.
Funding
Abstract
Regulated protein-protein interactions are critical for cell signaling, differentiation, and development. For the study of dynamic regulation of protein interactions in vivo, there is a need for techniques that can yield time-resolved information and probe multiple protein binding partners simultaneously, using small amounts of starting material. Here we describe a single-cell protein interaction assay. Single-cell lysates are generated at defined time points and analyzed using single-molecule pull-down, yielding information about dynamic protein complex regulation in vivo. We established the utility of this approach by studying PAR polarity proteins, which mediate polarization of many animal cell types. We uncovered striking regulation of PAR complex composition and stoichiometry during Caenorhabditis elegans zygote polarization, which takes place in less than 20 min. PAR complex dynamics are linked to the cell cycle by Polo-like kinase 1 and govern the movement of PAR proteins to establish polarity. Our results demonstrate an approach to study dynamic biochemical events in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.