ArticlePloS one2017
Classes 1 and 2 integrons in faecal Escherichia coli strains isolated from mother-child pairs in Nigeria.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Antimicrobial resistance in Enterobacterales infections among children in sub-Saharan Africa: a systematic review and meta-analysis.EClinicalMedicine · 2024Article
- Prevalence and molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolates from dairy cattle with endometritis in Gansu Province, China.BMC veterinary research · 2024Article
- Distribution diversity and expression regulation of class 1 integron promoters in clinical isolates ofFrontiers in microbiology · 2024Article
- Molecular Characterization of Resistance and Virulence Factors ofInfection and drug resistance · 2024Article
- Risk of sharing resistant bacteria and/or resistance elements between dogs and their owners.BMC veterinary research · 2022Article
- Detection of tem-1 and class-1 integrons in multidrug resistant uropathogens from HIV patients with asymptomatic bacteriuria in a Tertiary Care Hospital, SouthWest Nigeria.African health sciences · 2022Article
- Molecular characterisation of extended-spectrum ß-lactamase producing Escherichia coli in wild birds and cattle, Ibadan, Nigeria.BMC veterinary research · 2021Article
- Characterization of Integrons and Quinolone Resistance in ClinicalInternational journal of microbiology · 2021Article
- A plasmid-encoded papB paralogue modulates autoaggregation of Escherichia coli transconjugants.BMC research notes · 2020Article
- High diversity and variability of pipolins among a wide range of pathogenic Escherichia coli strains.Scientific reports · 2020Article
- Distribution of class 1 integrons in historic and contemporary collections of human pathogenic Escherichia coli.PloS one · 2020Article
- A large self-transmissible resistance plasmid from Nigeria contains genes that ameliorate a carrying cost.Scientific reports · 2019Article
- Prevalence of Integrons and Insertion Sequences in ESBL-ProducingInternational journal of environmental research and public health · 2018Article
- Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAntimicrobial resistance among enteric bacteria in Africa is increasingly mediated by integrons on horizontally acquired genetic elements. There have been recent reports of such elements in invasive pathogens across Africa, but very little is known about the faecal reservoir of integron-borne genes. METHODS AND
findingsWe screened 1098 faecal Escherichia coli isolates from 134 mother-child pairs for integron cassettes by PCR using primers that anneal to the 5' and 3' conserved ends of the cassette regions and for plasmid replicons. Genetic relatedness of isolates was determined by flagellin and multi-locus sequence typing. Integron cassettes were amplified in 410 (37.5%) isolates and were significantly associated with resistance to trimethoprim and multiple resistance. Ten cassette combinations were found in class 1 and two in class 2 integrons. The most common class 1 cassette configurations were single aadA1 (23.4%), dfrA7 (18.3%) and dfrA5 (14.4%). Class 2 cassette configurations were all either dfrA1-satI-aadA1 (n = 31, 7.6%) or dfrA1-satI (n = 13, 3.2%). A dfr cassette was detected in 294 (31.1%) of trimethoprim resistant strains and an aadA cassette in 242 (23%) of streptomycin resistant strains. Strains bearing integrons carried a wide range of plasmid replicons of which FIB/Y (n = 169; 41.2%) was the most frequently detected. Nine isolates from five different individuals carried the dfrA17-aadA5-bearing ST69 clonal group A (CGA). The same integron cassette combination was identified from multiple distinct isolates within the same host and between four mother-child pairs.
conclusionsIntegrons are important determinants of resistance in faecal E. coli. Plasmids in integron-containing strains may contribute to dispersing resistance genes. There is a need for improved surveillance for resistance and its mechanisms of dissemination and persistence and mobility of resistance genes in the community and clinical settings.
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