ArticleJournal of visualized experiments : JoVE2017
Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Interactions Between Inbreeding, Fitness and the Bacterial Microbiome inEvolutionary applications · 2026Article
- Semifield evaluation of autodissemination systems in Aedes notoscriptus and Culex quinquefasciatus and laboratory analysis of In2Care formulation effects.Journal of medical entomology · 2026Article
- Impact of larval diet on fitness outcomes of Aedes aegypti mosquitoes infected with wAlbB and wMelM.Parasites & vectors · 2025Article
- Fitness compatibility and dengue virus Inhibition in a Bangladeshi strain of Aedes aegypti infected with the Wolbachia strain wAlbB.Scientific reports · 2025Article
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- Evaluation of In2Care mosquito stations for suppression of the Australian backyard mosquito, Aedes notoscriptus (Diptera: Culicidae).Journal of medical entomology · 2023Article
- Fitness costs of Wolbachia shift in locally-adapted Aedes aegypti mosquitoes.Environmental microbiology · 2022Article
- Wolbachia inhibits ovarian formation and increases blood feeding rate in female Aedes aegypti.PLoS neglected tropical diseases · 2022Article
- Trash to Treasure: How Insect Protein and Waste Containers Can Improve the Environmental Footprint of Mosquito Egg Releases.Pathogens (Basel, Switzerland) · 2022Article
- A decade of stability for wMel Wolbachia in natural Aedes aegypti populations.PLoS pathogens · 2022Article
- Studies on the fitness characteristics ofFrontiers in microbiology · 2022Article
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- AApplied and environmental microbiology · 2021Article
- Infertility and fecundity loss of Wolbachia-infected Aedes aegypti hatched from quiescent eggs is expected to alter invasion dynamics.PLoS neglected tropical diseases · 2021Article
- Article
- Feeding and Quantifying Animal-Derived Blood and Artificial Meals in Aedes aegypti Mosquitoes.Journal of visualized experiments : JoVE · 2020Article
- Impacts of Low Temperatures on Wolbachia (Rickettsiales: Rickettsiaceae)-Infected Aedes aegypti (Diptera: Culicidae).Journal of medical entomology · 2020Article
- Endosymbiont diversity in natural populations of Tetranychus mites is rapidly lost under laboratory conditions.Heredity · 2020Article
- Persistent deleterious effects of a deleterious Wolbachia infection.PLoS neglected tropical diseases · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, chikungunya and Zika. Wolbachia-infected mosquitoes can be released into the field to either reduce population sizes through incompatible matings or to transform populations with mosquitoes that are refractory to virus transmission. For these strategies to succeed, the mosquitoes released into the field from the laboratory must be competitive with native mosquitoes. However, maintaining mosquitoes in the laboratory can result in inbreeding, genetic drift and laboratory adaptation which can reduce their fitness in the field and may confound the results of experiments. To test the suitability of different Wolbachia infections for deployment in the field, it is necessary to maintain mosquitoes in a controlled laboratory environment across multiple generations. We describe a simple protocol for maintaining Ae. aegypti mosquitoes in the laboratory, which is suitable for both Wolbachia-infected and wild-type mosquitoes. The methods minimize laboratory adaptation and implement outcrossing to increase the relevance of experiments to field mosquitoes. Additionally, colonies are maintained under optimal conditions to maximize their fitness for open field releases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.