ArticleNano letters2017
Ionic Current-Based Mapping of Short Sequence Motifs in Single DNA Molecules Using Solid-State Nanopores.
Article in Nano letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Article
- Preparation and Detection of Designed DNA Carriers for Nanopore Sensing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- A genetic circuit on a single DNA molecule as an autonomous dissipative nanodevice.Nature communications · 2024Article
- DNA Barcodes Using a Dual Nanopore Device.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Discriminating protein tags on a dsDNA construct using a Dual Nanopore Device.Scientific reports · 2022Article
- Electronic Mapping of a Bacterial Genome with Dual Solid-State Nanopores and Active Single-Molecule Control.ACS nano · 2022Article
- Ionic current magnetic fields in 3D finite-length nanopores and nanoslits.European physical journal plus · 2022Article
- DNA Labeling Using DNA Methyltransferases.Advances in experimental medicine and biology · 2022Article
- Structure-flexible DNA origami translocation through a solid-state nanopore.RSC advances · 2021Article
- Article
- Sequence-Specific Detection of DNA Strands Using a Solid-State Nanopore Assisted by Microbeads.Micromachines · 2020Article
- Review
- Channel from bacterial virus T7 DNA packaging motor for the differentiation of peptides composed of a mixture of acidic and basic amino acids.Biomaterials · 2019Article
- Simple and Efficient Room-Temperature Release of Biotinylated Nucleic Acids from Streptavidin and Its Application to Selective Molecular Detection.Analytical chemistry · 2019Article
- Repurposing enzymatic transferase reactions for targeted labeling and analysis of DNA and RNA.Current opinion in biotechnology · 2019Review
- Solid-state nanopore hydrodynamics and transport.Biomicrofluidics · 2019Article
- Active Delivery of Single DNA Molecules into a Plasmonic Nanopore for Label-Free Optical Sensing.Nano letters · 2018Article
- Review
- A general strategy for direct, enzyme-catalyzed conjugation of functional compounds to DNA.Nucleic acids research · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanopore sensors show great potential for rapid, single-molecule determination of DNA sequence information. Here, we develop an ionic current-based method for determining the positions of short sequence motifs in double-stranded DNA molecules with solid-state nanopores. Using the DNA-methyltransferase M.TaqI and a biotinylated S-adenosyl-l-methionine cofactor analogue we create covalently attached biotin labels at 5'-TCGA-3' sequence motifs. Monovalent streptavidin is then added to bind to the biotinylated sites giving rise to additional current blockade signals when the DNA passes through a conical quartz nanopore. We determine the relationship between translocation time and position along the DNA contour and find a minimum resolvable distance between two labeled sites of ∼200 bp. We then characterize a variety of DNA molecules by determining the positions of bound streptavidin and show that two short genomes can be simultaneously detected in a mixture. Our method provides a simple, generic single-molecule detection platform enabling DNA characterization in an electrical format suited for portable devices for potential diagnostic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.