Evidence map›Paper›PMID 28828672›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2017

Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles.

Chris Wang, Austin Wang, Fang Wei, David T W Wong, Michael Tu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
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  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Chris WangSchool of Dentistry, University of California, Los Angeles, CA, USA.
Austin WangSchool of Dentistry, University of California, Los Angeles, CA, USA.
Fang WeiSchool of Dentistry, University of California, Los Angeles, CA, USA.
David T W WongSchool of Dentistry, University of California, Los Angeles, CA, USA.
Michael TuSchool of Dentistry, University of California, Los Angeles, CA, USA. emtu@ucla.edu.

Funding

UCLA Dentist-Scientist and Oral Health-Researcher Training ProgramT90DE022734 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WONG, DAVID T · 2012 to 2017
$2.3M
NIDCR NIH HHS T90 DE022734
6 · The paper itself

Abstract

In order to more fully elucidate the biogenesis of exosomes, a type of extracellular vesicles (EVs) and understand the role of EVs in disease processes, it is necessary to develop methods to capture EVs and induce the unloading of viable cargo. Traditionally, ultracentrifugation followed by chemical based EV lysis techniques is used to isolate these extracellular vesicles and release their internal content. Here, we describe a novel technique for capturing and releasing exosomal content through magnetic bead-based EV extraction coupled with electric field induced release and measurement (EFIRM). The usage of low-voltage electric fields allows the EV to be lysed without chemical treatment, and surface immobilized probes can allow for the rapid capture of the content of lysed EVs. EFIRM as an integrated EV lysing and analysis system offers great potential for the investigation of EVs in clinical and basic science contexts.

Indexed as

BiomarkersElectricityMolecular Diagnostic TechniquesExosomesExtracellular VesiclesFluorescent Antibody TechniqueHumansImmunomagnetic SeparationRadioimmunoprecipitation AssayUltracentrifugationBiomarkersBiomarkersDiagnosticsElectric field induced release and measurement (EFIRM)ExosomesExtracellular vesicles

Identifiers

PMID28828672
PMCPMC6937371

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.