ArticlePeerJ2017
Freeze-thaw decellularization of the trabecular meshwork in an
Article in PeerJ, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Vascularized Cardiac Tissue Engineering: From Advances in Biofabrication to Translational Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Decellularization method and tissue region impact the structural properties and composition of porcine vaginal extracellular matrix.Acta biomaterialia · 2026Article
- F4H5 Alleviates Increased Outflow Resistance Induced by Emulsified Silicone Oil In Vitro.Investigative ophthalmology & visual science · 2025Article
- Development of artificial bone graft viaMaterials today. Bio · 2023Article
- Allogenic Bone Graft in Dentistry: A Review of Current Trends and Developments.International journal of molecular sciences · 2023Review
- Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Article
- Trabeculopuncture as a predictive test of distal outflow resistance in canal-based surgery.Scientific reports · 2022Article
- An Optimized Method to Decellularize Human Trabecular Meshwork.Bioengineering (Basel, Switzerland) · 2022Article
- Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering.Bioactive materials · 2022Review
- Efficient Decellularization by Application of Moderate High Hydrostatic Pressure with Supercooling Pretreatment.Micromachines · 2021Article
- Interactions at engineered graft-tissue interfaces: A review.APL bioengineering · 2020Review
- Decellularizing the Porcine Optic Nerve Head: Toward a Model to Study the Mechanobiology of Glaucoma.Translational vision science & technology · 2020Article
- Intraocular pressure reduction in a pigmentary glaucoma model by Goniotome Ab interno trabeculectomy.PloS one · 2020Article
- Generating cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies.Methods in cell biology · 2020Article
- Outflow facility and extent of angle closure in a porcine model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- RKI-1447, a Rho kinase inhibitor, causes ocular hypotension, actin stress fiber disruption, and increased phagocytosis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2019Article
- Structure-Function Changes of the Porcine Distal Outflow Tract in Response to Nitric Oxide.Investigative ophthalmology & visual science · 2018Article
- Outflow enhancement by three different ab interno trabeculectomy procedures in a porcine anterior segment model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2018Article
- High-Resolution, Three-Dimensional Reconstruction of the Outflow Tract Demonstrates Segmental Differences in Cleared Eyes.Investigative ophthalmology & visual science · 2018Article
- A porcine ex vivo model of pigmentary glaucoma.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
objectiveThe trabecular meshwork (TM) is the primary substrate of outflow resistance in glaucomatous eyes. Repopulating diseased TM with fresh, functional TM cells might be a viable therapeutic approach. Decellularized TM scaffolds have previously been produced by ablating cells with suicide gene therapy or saponin, which risks incomplete cell removal or dissolution of the extracellular matrix, respectively. We hypothesized that improved trabecular meshwork cell ablation would result from freeze-thaw cycles compared to chemical treatment. MATERIALS AND
methodsWe obtained 24 porcine eyes from a local abattoir, dissected and mounted them in an anterior segment perfusion within two hours of sacrifice. Intraocular pressure (IOP) was recorded continuously by a pressure transducer system. After 72 h of IOP stabilization, eight eyes were assigned to freeze-thaw (F) ablation (-80 °C × 2), to 0.02% saponin (S) treatment, or the control group (C), respectively. The TM was transduced with an eGFP expressing feline immunodeficiency viral (FIV) vector and tracked via fluorescent microscopy to confirm ablation. Following treatment, the eyes were perfused with standard tissue culture media for 180 h. TM histology was assessed by hematoxylin and eosin staining. TM viability was evaluated by a calcein AM/propidium iodide (PI) assay. The TM extracellular matrix was stained with Picro Sirius Red. We measured IOP and modeled it with a linear mixed effects model using a B-spline function of time with five degrees of freedom.
resultsF and S experienced a similar IOP reduction of 30% from baseline (
conclusionWe developed a rapid TM ablation method that uses cyclic freezing that is free of biological or chemical agents and able to produce a decellularized TM scaffold with preserved TM extracellular matrix in an organotypic perfusion culture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.