ArticleScientific reports2017
Pre-culture Sudan Black B treatment suppresses autofluorescence signals emitted from polymer tissue scaffolds.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
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- Integrated approach to cell growth and recovery in silk fibroin scaffolds via a spin-down system.Biomaterials and biosystems · 2025Article
- Hydrogen-Bond Cross-Linking between Chitosan and Urethane-Modified Polycaprolactone: Influence of PCL Structure on Cryogel Properties.ACS biomaterials science & engineering · 2025Article
- Quantitative investigation of photobleaching-based autofluorescence suppression in formalin-fixed paraffin-embedded human tissue.Biomedical optics express · 2025Article
- Extrusion-Based Printing of Myoblast-Loaded Fibrin Microthreads to Induce Myogenesis.Journal of functional biomaterials · 2025Article
- An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence.PloS one · 2024Article
- Sudan Black B Pretreatment to Suppress Autofluorescence in Silk Fibroin Scaffolds.ACS biomaterials science & engineering · 2023Article
- Blocking autofluorescence in brain tissues affected by ischemic stroke, hemorrhagic stroke, or traumatic brain injury.Frontiers in immunology · 2023Article
- 3D Polymer Architectures for the Identification of Optimal Dimensions for Cellular Growth of 3D Cellular Models.Polymers · 2022Article
- Development of an Oriented Co-Culture System Using 3D Scaffolds Fabricated via Non-Linear Lithography.Materials (Basel, Switzerland) · 2022Article
- A Fibrin Coating Method of Polypropylene Meshes Enables the Adhesion of Menstrual Blood-Derived Mesenchymal Stromal Cells: A New Delivery Strategy for Stem Cell-Based Therapies.International journal of molecular sciences · 2021Article
- DNA-DAPI Interaction-Based Method for Cell Proliferation Rate Evaluation in 3D Structures.Current issues in molecular biology · 2021Article
- The Wall-Associated Receptor-Like Kinase TaWAK7D Is Required for Defense Responses toInternational journal of molecular sciences · 2021Article
- Biocompatible Electrospun Polycaprolactone-Polyaniline Scaffold Treated with Atmospheric Plasma to Improve Hydrophilicity.Bioengineering (Basel, Switzerland) · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The wheat LLM-domain-containing transcription factor TaGATA1 positively modulates host immune response to Rhizoctonia cerealis.Journal of experimental botany · 2020Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In tissue engineering, autofluorescence of polymer scaffolds often lowers the image contrast, making it difficult to examine cells and subcellular structures. Treating the scaffold materials with Sudan Black B (SBB) after cell fixation can effectively suppress autofluorescence, but this approach is not conducive to live cell imaging. Post-culture SBB treatment also disrupts intracellular structures and leads to reduced fluorescence intensity of the targets of interest. In this study, we introduce pre-culture SBB treatment to suppress autofluorescence, where SBB is applied to polymeric scaffold materials before cell seeding. The results show that the autofluorescence signals emitted from polycaprolactone (PCL) scaffolds in three commonly used fluorescence channels effectively decrease without diminishing the fluorescence signals emitted from the cells. The pre-culture SBB treatment does not significantly affect cell viability. The autofluorescence suppressive effect does not substantially diminish during the culturing period up to 28 days. The results also show that cell migration, proliferation, and myogenic differentiation in pre-culture SBB-treated groups do not exhibit statistical difference from the non-treated groups. As such, this approach greatly improves the fluorescence image quality for examining live cell behaviors and dynamics while the cells are cultured within autofluorescent polymer scaffolds.
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