ArticleDevelopment (Cambridge, England)2017
A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport.
Article in Development (Cambridge, England), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Regulation and function of specialized membrane protrusions in intercellular communication.Nature reviews. Molecular cell biology · 2026Review
- Emerging Roles of Cytoneme-Mediated Signaling in Cancer.International journal of molecular sciences · 2026Review
- Ovarian germline stem cell dedifferentiation is cytoneme dependent.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.Development (Cambridge, England) · 2024Article
- Article
- Hedgehog signaling guides migration of primordial germ cells to the Drosophila somatic gonad.Genetics · 2023Article
- Cancer-associated fibroblasts influence Wnt/PCP signaling in gastric cancer cells by cytoneme-based dissemination of ROR2.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Non-Classical Intercellular Communications: Basic Mechanisms and Roles in Biology and Medicine.International journal of molecular sciences · 2023Review
- Review
- The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer.eLife · 2022Article
- Fixation of Embryonic Mouse Tissue for Cytoneme Analysis.Journal of visualized experiments : JoVE · 2022Article
- DCHS1, Lix1L, and the Septin Cytoskeleton: Molecular and Developmental Etiology of Mitral Valve Prolapse.Journal of cardiovascular development and disease · 2022Article
- Regulatory mechanisms of cytoneme-based morphogen transport.Cellular and molecular life sciences : CMLS · 2022Review
- Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations.Cells · 2021Review
- Article
- Article
- Polarized sorting of Patched enables cytoneme-mediated Hedgehog reception in the Drosophila wing disc.The EMBO journal · 2020Article
- Light-Inducible Generation of Membrane Curvature in Live Cells with Engineered BAR Domain Proteins.ACS synthetic biology · 2020Article
- Identification of Heparan-Sulfate Rich Cells in the Loose Connective Tissues of the Axolotl (Ambystoma mexicanum) with the Potential to Mediate Growth Factor Signaling during Regeneration.Regenerative engineering and translational medicine · 2020Article
- Dispatching Sonic Hedgehog: Molecular Mechanisms Controlling Deployment.Trends in cell biology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
During development, extracellular cues guiding cell fate determination are provided by morphogens. One mechanism by which morphogens are proposed to traverse extracellular space is by traveling along specialized filopodia called cytonemes. These cellular highways extend between signal-producing and -receiving cells to enable direct morphogen delivery. Although genetic studies support cytoneme involvement in morphogen transport, mechanistic insight into how they are regulated is limited owing to technical challenges associated with performing cell biological analysis of the delicate filopodial structures. Here, we introduce a fixation method whereby cultured cell cytonemes can be preserved for imaging studies, allowing investigation of cytoneme regulation using standard cell biological techniques. Using this method, we examined Hedgehog-containing cytonemes and identified a role for the Hedgehog deployment protein Dispatched in cytoneme stabilization. We demonstrate that Hedgehog and Dispatched colocalize in cytonemes, and that cholesterol-modified Hedgehog acts through Dispatched to increase cytoneme occurrence. Live imaging suggests that this occurs through Dispatched-mediated slowing of cytoneme retraction rates. Dispatched-induced cytoneme modulation was recapitulated in wing imaginal discs of transgenic
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.