ArticleExperimental eye research2017
Unravelling the stromal-nerve interactions in the human diabetic cornea.
Article in Experimental eye research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Novel full-thickness biomimetic corneal model for studying pathogenesis and treatment of diabetic keratopathy.Materials today. Bio · 2025Article
- Corneal epithelial-stromal constructs to study differences associated with diabetes mellitus.Experimental eye research · 2024Article
- Effects of hypoxia in the diabetic corneal stroma microenvironment.Experimental eye research · 2024Article
- Cell sheet-based approach to study the diabetic corneal stroma.Experimental eye research · 2023Article
- Monocarboxylate Transporters: Role and Regulation in Corneal Diabetes.Analytical cellular pathology (Amsterdam) · 2022Article
- Corneal Epithelial-Stromal Fibroblast Constructs to Study Cell-Cell Communication in Vitro.Bioengineering (Basel, Switzerland) · 2019Article
- Diabetes inhibits corneal epithelial cell migration and tight junction formation in mice and human via increasing ROS and impairing Akt signaling.Acta pharmacologica Sinica · 2019Article
- Corneal pain and experimental model development.Progress in retinal and eye research · 2019Review
- Influence of diabetes mellitus on anterior segment of the eye.Clinical interventions in aging · 2019Review
- Corneal alteration and pathogenesis in diabetes mellitus.International journal of ophthalmology · 2019Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Corneal defects due to diabetes mellitus (DM) may cause severe vision impairments. Current studies focus on the corneal epithelium and nerve defects neglecting the corneal stroma. The aim of this study was to develop a 3D in vitro model to examine the interactions between corneal stroma and nerves in the context of DM. Primary human corneal stromal fibroblasts isolated from healthy (HCFs), Type 1 (T1DM) and Type 2 (T2DM) patients were stimulated with stable ascorbic acid to secrete and assemble an extracellular matrix (ECM). Human neuronal cells were then seeded on top and differentiated to create the 3D co-cultures. Our data revealed successful co-culture of stromal fibroblasts and neuronal cells with large elongated neuron extensions. T2DM showed significant upregulation of Collagen III and IGF1 when compared to T1DM. Interestingly, upon nerve addition, those markers returned to HCF levels. Neuronal markers were also differentially modulated with T2DM co-cultures expressing high levels of βIII tubulin where T1DM co-cultures expressed Substance P. . Overall, our unique 3D co-culture model provides us with a tool that can be utilized for both molecular and therapeutic studies for diabetic keratopathy.
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Registered trials
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