ArticleMolecular and cellular biochemistry2018
Functional validation of ATF4 and GADD34 in Neuro2a cells by CRISPR/Cas9-mediated genome editing.
Article in Molecular and cellular biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Transcriptional regulation of solute carrier family 6 member 9 gene.Molecular biology reports · 2025Article
- Alleviation of liver fibrosis by inhibiting a non-canonical ATF4-regulated enhancer program in hepatic stellate cells.Nature communications · 2025Article
- Elucidation of OSW-1-Induced Stress Responses in Neuro2a Cells.International journal of molecular sciences · 2023Article
- Expression analysis and functional characterization of thioredoxin domain-containing protein 11.Molecular biology reports · 2022Article
- Elucidation of brefeldin A-induced ER and Golgi stress responses in Neuro2a cells.Molecular and cellular biochemistry · 2021Article
- Molecular characterization of mouse CREB3 regulatory factor in Neuro2a cells.Molecular biology reports · 2021Article
- Comparative Analysis of CREB3 and CREB3L2 Protein Expression in HEK293 Cells.International journal of molecular sciences · 2021Article
- Transcriptional regulation of the ER stress-inducible gene Sec16B in Neuro2a cells.Molecular and cellular biochemistry · 2021Article
- Characterization of IRE1α in Neuro2a cells by pharmacological and CRISPR/Cas9 approaches.Molecular and cellular biochemistry · 2020Article
- Elucidating post-translational regulation of mouse CREB3 in Neuro2a cells.Molecular and cellular biochemistry · 2018Article
- Expression analysis and functional characterization of the mouse cysteine-rich with EGF-like domains 2.Scientific reports · 2018Article
- Application of NanoBiT for Monitoring Dimerization of the Null Hong Kong Variant of α-1-Antitrypsin, NHK, in Living Cells.Molecular biotechnology · 2018Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Activating transcription factor 4 (ATF4), which is ubiquitously expressed, plays a crucial role in regulating various stress-responsive genes under pathophysiological conditions. Further, growth arrest and DNA damage-inducible gene 34 (GADD34), a downstream target of ATF4, has been reported to negatively regulate ATF4 expression. To understand the relationship between intrinsic ATF4 and GADD34 under resting and ER stress conditions, we used a novel gene editing approach, CRISPR/Cas9, to integrate antibiotic-resistant genes into the target genes, ATF4 and GADD34. First, we manipulated the ATF4 gene in the mouse neuroblastoma cell line, Neuro2a, and compared the ER stress responses between parental and ATF4-edited Neuro2a cells. Next, we established Neuro2a cells with edited GADD34 and ATF4/GADD34 genes and found that ATF4 acts as a proapoptotic factor, but GADD34 depletion did not attenuate the expression of cleaved caspase-3 induced by tunicamycin treatment. These findings provide new insights into the ATF4 signaling cascades. Additionally, the rapid establishment of cells lacking multiple genes using this CRISPR/Cas9 system will be a powerful tool for exploring various cellular issues under pathophysiological conditions.
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