ArticleACS biomaterials science & engineering2017
Predehydration and Ice Seeding in the Presence of Trehalose Enable Cell Cryopreservation.
Article in ACS biomaterials science & engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 81 citations in OpenAlex.
- Cryopreservable, scalable and ready-to-use cell-laden patches for diabetic ulcer treatment.Bioactive materials · 2025Article
- Proteomic approach for evaluating cryoprotectant formulations for enhanced post-cryopreservation recovery of yeast.Scientific reports · 2025Article
- Controlled Ice Nucleation With a Sand-PDMS Film Device Enhances Cryopreservation of Mouse Preantral Ovarian Follicles.Journal of medical devices · 2024Article
- Sucrose versus Trehalose: Observations from Comparative Study Using Molecular Dynamics Simulations.ACS omega · 2024Article
- Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities.RSC medicinal chemistry · 2024Review
- Supercooled preservation of cultured primary rat hepatocyte monolayers.Frontiers in bioengineering and biotechnology · 2024Article
- Core-Shell Microfiber Encapsulation Enables Glycerol-Free Cryopreservation of RBCs with High Hematocrit.Nano-micro letters · 2023Article
- Advanced cryopreservation engineering strategies: the critical step to utilize stem cell products.Cell regeneration (London, England) · 2023Review
- A highly active mineral-based ice nucleating agent supportsJournal of the Royal Society, Interface · 2023Article
- Incorporate delivery, warming and washing methods into efficient cryopreservation.Frontiers in bioengineering and biotechnology · 2023Article
- Article
- Human Ovarian Follicles Xenografted in Immunoisolating Capsules Survive Long Term Implantation in Mice.Frontiers in endocrinology · 2022Article
- Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells.Bioactive materials · 2021Article
- Effects of storage media, supplements and cryopreservation methods on quality of stem cells.World journal of stem cells · 2021Review
- Cryomicroneedles for transdermal cell delivery.Nature biomedical engineering · 2021Article
- Advanced technologies for the preservation of mammalian biospecimens.Nature biomedical engineering · 2021Review
- Review
- Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Cryopreservation of Mesenchymal Stem Cells Using Medical Grade Ice Nucleation Inducer.International journal of molecular sciences · 2020Article
- Non-aqueous, zwitterionic solvent as an alternative for dimethyl sulfoxide in the life sciences.Communications chemistry · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Conventional approaches for cell cryopreservation require the use of toxic membrane-penetrating cryoprotective agents (pCPA), which limits the clinical application of cryopreserved cells. Here, we show intentionally induced ice formation at a high subzero temperature (> -10 °C) during cryopreservation, which is often referred to as ice seeding, could result in significant cell injury in the absence of any pCPA. This issue can be mitigated by predehydrating cells using extracellular trehalose to their minimal volume with minimized osmotically active water before ice seeding. We further observe that ice seeding can minimize the interfacial free energy that drives the devastating ice recrystallization-induced cell injury during warming cryopreserved samples. Indeed, by combining predehydration using extracellular trehalose with ice seeding at high subzero temperatures, high cell viability or recovery is achieved for fibroblasts, adult stem cells, and red blood cells after cryopreservation without using any pCPA. The pCPA-free technology developed in this study may greatly facilitate the long-term storage and ready availability of living cells, tissues, and organs that are of high demand by modern cell-based medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.