Evidence map›Paper›PMID 28824873›Full record

ArticleFrontiers in oncology2017

Functional Assay of Cancer Cell Invasion Potential Based on Mechanotransduction of Focused Ultrasound.

Andrew C Weitz, Nan Sook Lee, Chi Woo Yoon, Adrineh Bonyad, Kyo Suk Goo, Seaok Kim, Sunho Moon, Hayong Jung, Qifa Zhou, Robert H Chow and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in oncology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.8field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 21 citations in OpenAlex.

  1. Sono-Mechanogenetics: Linking Ultrasound Physics With Cellular Mechanobiology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
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  13. Low-Intensity Ultrasound Modulates CaAnnals of biomedical engineering · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Andrew C WeitzUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Nan Sook LeeUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Chi Woo YoonUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Adrineh BonyadZilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA, United States.
Kyo Suk GooUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Seaok KimUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Sunho MoonUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Hayong JungUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Qifa ZhouUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
Robert H ChowZilkha Neurogenetic Institute, University of Southern California, Los Angeles, CA, United States.
K Kirk ShungUltrasonic Transducer Resource Center, University of Southern California, Los Angeles, CA, United States.
University of Southern California · USSouthern California Eye Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer cells undergo a number of biophysical changes as they transform from an indolent to an aggressive state. These changes, which include altered mechanical and electrical properties, can reveal important diagnostic information about disease status. Here, we introduce a high-throughput, functional technique for assessing cancer cell invasion potential, which works by probing for the mechanically excitable phenotype exhibited by invasive cancer cells. Cells are labeled with fluorescent calcium dye and imaged during stimulation with low-intensity focused ultrasound, a non-contact mechanical stimulus. We show that cells located at the focus of the stimulus exhibit calcium elevation for invasive prostate (PC-3 and DU-145) and bladder (T24/83) cancer cell lines, but not for non-invasive cell lines (BPH-1, PNT1A, and RT112/84). In invasive cells, ultrasound stimulation initiates a calcium wave that propagates from the cells at the transducer focus to other cells, over distances greater than 1 mm. We demonstrate that this wave is mediated by extracellular signaling molecules and can be abolished through inhibition of transient receptor potential channels and inositol trisphosphate receptors, implicating these proteins in the mechanotransduction process. If validated clinically, our technology could provide a means to assess tumor invasion potential in cytology specimens, which is not currently possible. It may therefore have applications in diseases such as bladder cancer, where cytologic diagnosis of tumor invasion could improve clinical decision-making.

Indexed as

bladder cancercalcium imagingcancer invasionfocused ultrasoundprostate cancer

Identifiers

PMID28824873
PMCPMC5545605
OpenAlexW2742912367

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.