Evidence map›Paper›PMID 28823107›Full record

ArticleJournal of fluorescence2017

Fluorescent Labeling of Protein Using Blue-Emitting 8-Amino-BODIPY Derivatives.

Dokyoung Kim, Donghee Ma, Muwoong Kim, Yuna Jung, Na Hee Kim, Chiho Lee, Seo Won Cho, Sungnam Park, Youngbuhm Huh, Junyang Jung and 1 more

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Article in Journal of fluorescence, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Acta crystallographica. Section F, Structural biology communications · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Dokyoung KimDepartment of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea. dkim@khu.ac.kr.ORCID http://orcid.org/0000-0002-7756-3560
Donghee MaDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, 37673, South Korea.
Muwoong KimDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, South Korea.
Yuna JungDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, South Korea.
Na Hee KimDepartment of Biomedical Science, Graduate School, Kyung Hee University, Seoul, 02447, South Korea.
Chiho LeeDepartment of Chemistry, Korea University, Seoul, 02841, South Korea.
Seo Won ChoDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, 37673, South Korea.
Sungnam ParkDepartment of Chemistry, Korea University, Seoul, 02841, South Korea.
Youngbuhm HuhDepartment of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea.
Junyang JungDepartment of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, Seoul, 02447, South Korea.
Kyo Han AhnDepartment of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyungbuk, 37673, South Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

8-Amino-BODIPY (boron-dipyrromethane) dyes show bright blue fluorescence. Disclosed here are synthesis and characterization of the photophysical properties of a series of functionalized 8-Amino-BODIPY (BP1-4) for protein labeling. The compact structure and solvent-insensitive absorption property of the dye are desirable features for protein labeling. For the model protein, bovine serum albumin (BSA), the labeling proceeds under mild condition via amide bond formation or thiol-ene conjugation with maintaining the bright blue fluorescence. The chromatography and mass spectroscopy analysis clearly support the labeling of the BODIPY dye on the BSA. The protein labeling with blue-emitting BODIPY would be applicable for studying protein dynamics and fluorescence resonance energy transfer (FRET) with intrinsic biomolecules.

Indexed as

FluorescenceAnimalsBoron CompoundsCattleFluorescence Resonance Energy TransferFluorescent DyesModels, MolecularSerum Albumin, Bovine4,4-difluoro-4-bora-3a,4a-diaza-s-indaceneBoron CompoundsFluorescent DyesSerum Albumin, BovineBioconjugationBlue-emitting BODIPYFluorescent labelingProtein labeling

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.