XCC2366, A Gene Encoding A Putative TetR Family Transcriptional Regulator, is Required for Acriflavin Resistance and Virulence of Xanthomonas campestris pv. campestris.

Chao-Tsai Liao, Hsueh-Hsia Lo, Weng-Ting Peng, Wan-Ling Song, Shin-Chiao Du, Yi-Min Hsiao

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Article in Current microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Chao-Tsai LiaoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Hsueh-Hsia LoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Weng-Ting PengDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Wan-Ling SongDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Shin-Chiao DuDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan.

Yi-Min HsiaoDepartment of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, 406, Taiwan. ymhsiao@ctust.edu.tw.

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6 · The paper itself

Abstract

Xanthomonas campestris pv. campestris (Xcc) is the phytopathogen that causes black rot disease in cruciferous plants. The XCC2366 gene product is annotated as a protein belonging to the TetR family of transcriptional regulators. In this study, we evaluated the function and expression of the XCC2366 gene. Mutational analysis demonstrated that XCC2366 is involved in the resistance to acriflavin and is necessary for virulence in Xcc. In addition, the XCC2366 transcription initiation site was mapped at nucleotide A, 63 nucleotide upstream of the XCC2366 translation start codon. Furthermore, transcriptional analysis revealed that the expression of XCC2366 is induced in the presence of acriflavin. Reporter assay also showed that XCC2366 regulates its own expression under acriflavin-supplemented condition. To the best of our knowledge, acriflavin resistance-related gene in the crucifer pathogen Xcc was characterized for the first time.

Indexed as

Drug Resistance, BacterialAcriflavineAnti-Bacterial AgentsDNA Mutational AnalysisGene Expression ProfilingGene Expression Regulation, BacterialPlant DiseasesTranscription FactorsTranscription Initiation SiteVirulenceVirulence FactorsXanthomonas campestrisAcriflavineAnti-Bacterial AgentsTranscription FactorsVirulence Factors

Identifiers

PMID28821943

DOI10.1007/s00284-017-1328-5

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