ArticleScientific reports2017
Three recombinantly expressed apple tyrosinases suggest the amino acids responsible for mono- versus diphenolase activity in plant polyphenol oxidases.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 88 citations in OpenAlex.
- In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols.FEBS open bio · 2026Article
- Investigation and characterization of the GhPPO gene family and its expression in response to fibre cell growth and biotic stress in Gossypium hirsutum L.BMC genomics · 2025Article
- Gene Expression and Enzyme Kinetics of Polyphenol Oxidases in Strawberry and Their Possible Involvement in Enzymatic Browning Reactions in Strawberry Nectar.Foods (Basel, Switzerland) · 2025Article
- Human recombinant tyrosinase destabilization caused by the double mutation R217Q/R402Q.Protein science : a publication of the Protein Society · 2025Article
- Fine mapping and identification of the geneFrontiers in plant science · 2025Article
- Characterization of two bacterial tyrosinases from the halophilic bacterium Hahella sp. CCB MM4 relevant for phenolic compounds oxidation in wetlands.FEBS open bio · 2024Article
- Extraction, Purification, and Characterization of Olive (Journal of agricultural and food chemistry · 2024Article
- Tyrosinases: a family of copper-containing metalloenzymes.Chemtexts · 2024Article
- Biochemical Investigations of Five Recombinantly Expressed Tyrosinases Reveal Two Novel Mechanisms Impacting Carbon Storage in Wetland Ecosystems.Environmental science & technology · 2023Article
- Genome-Wide Analysis of the Polyphenol Oxidase Gene Family inAntioxidants (Basel, Switzerland) · 2023Article
- Contribution of the Tyrosinase (MoTyr) to Melanin Synthesis, Conidiogenesis, Appressorium Development, and Pathogenicity inJournal of fungi (Basel, Switzerland) · 2023Article
- Olive Polyphenol Oxidase Gene Family.International journal of molecular sciences · 2023Article
- Biochemical characterization of Dimocarpus longan polyphenol oxidase provides insights into its catalytic efficiency.Scientific reports · 2022Article
- The Infestation of Olive Fruits byMolecules (Basel, Switzerland) · 2022Article
- Polyphenol oxidase and enzymatic browning in apricot (Current research in food science · 2022Article
- PbLAC4-like, activated by PbMYB26, related to the degradation of anthocyanin during color fading in pear.BMC plant biology · 2021Article
- Molecular and Enzymatic Characterization of Flavonoid 3'-Hydroxylase ofPlants (Basel, Switzerland) · 2021Article
- Polyphenol-Hydroxylating Tyrosinase Activity under Acidic pH Enables Efficient Synthesis of Plant Catechols and Gallols.Microorganisms · 2021Article
- Similar but Still Different: Which Amino Acid Residues Are Responsible for Varying Activities in Type-III Copper Enzymes?Chembiochem : a European journal of chemical biology · 2021Review
- Identification of Amino Acid Residues Responsible for C-H Activation in Type-III Copper Enzymes by Generating Tyrosinase Activity in a Catechol Oxidase.Angewandte Chemie (International ed. in English) · 2020Article
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4 authors at 1 institution in 1 country.
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Abstract
Tyrosinases and catechol oxidases belong to the polyphenol oxidase (PPO) enzyme family, which is mainly responsible for the browning of fruits. Three cDNAs encoding PPO pro-enzymes have been cloned from leaves of Malus domestica (apple, MdPPO). The three pro-enzymes MdPPO1-3 were heterologously expressed in E. coli yielding substantial amounts of protein and have been characterized with regard to their optimum of activity resulting from SDS, acidic and proteolytic activation. Significant differences were found in the kinetic characterization of MdPPO1-3 when applying different mono- and diphenolic substrates. All three enzymes have been classified as tyrosinases, where MdPPO1 exhibits the highest activity with tyramine (k
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