ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2017
Transcriptomic Analysis of Ribosome-Bound mRNA in Cortical Neurites
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 60 citations in OpenAlex.
- Locally synthesized glycyl aminoacyl-tRNA synthetase is important for local translation in neurons.Life science alliance · 2026Article
- The choreography of development: RNA localization across time and space.Development (Cambridge, England) · 2026Review
- In Vivo Massively Parallel Reporter Assay Reveals Sequence Determinants of mRNA Localization in Astrocytes.bioRxiv : the preprint server for biology · 2026Article
- Simultaneous profiling of native-state proteomes and transcriptomes of neural cell types using proximity labeling.Nature communications · 2026Article
- Translational Remodeling of the Synaptic Proteome During Aging.Aging cell · 2025Article
- Excitatory-neuron-derived interleukin-34 supports cortical developmental microglia function.Immunity · 2025Article
- Experience-Dependent Intrinsic Plasticity in Layer IV of Barrel Cortex at Whisking Onset.eNeuro · 2025Article
- Immediately following the onset of active whisking, the input layer of barrel cortex exhibits a transient period of increased excitability that depends on prior experience.bioRxiv : the preprint server for biology · 2025Article
- The Control of Cortical Folding: Multiple Mechanisms, Multiple Models.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2024Review
- The intersection of sleep and synaptic translation in synaptic plasticity deficits in neurodevelopmental disorders.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Review
- RNA in axons, dendrites, synapses and beyond.Frontiers in molecular neuroscience · 2024Review
- The brain's dark transcriptome: Sequencing RNA in distal compartments of neurons and glia.Current opinion in neurobiology · 2023Review
- Local translation in microglial processes is required for efficient phagocytosis.Nature neuroscience · 2023Article
- Non-muscle myosins control radial glial basal endfeet to mediate interneuron organization.PLoS biology · 2023Article
- Effects of alcohol and PARP inhibition on RNA ribosomal engagement in cortical excitatory neurons.Frontiers in molecular neuroscience · 2023Article
- Activity-dependent translation dynamically alters the proteome of the perisynaptic astrocyte process.Cell reports · 2022Article
- A Proposed Role for Interactions between Argonautes, miRISC, and RNA Binding Proteins in the Regulation of Local Translation in Neurons and Glia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Review
- Subcellular and regional localization of mRNA translation in midbrain dopamine neurons.Cell reports · 2022Article
- The translatome of neuronal cell bodies, dendrites, and axons.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Neuronal ribosomes exhibit dynamic and context-dependent exchange of ribosomal proteins.Nature communications · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Localized translation in neurites helps regulate synaptic strength and development. Dysregulation of local translation is associated with many neurological disorders. However, due to technical limitations, study of this phenomenon has largely been limited to brain regions with laminar organization of dendrites such as the hippocampus or cerebellum. It has not been examined in the cortex, a region of importance for most neurological disorders, where dendrites of each neuronal population are densely intermingled with cell bodies of others. Therefore, we have developed a novel method, SynapTRAP, which combines synaptoneurosomal fractionation with translating ribosome affinity purification to identify ribosome-bound mRNA in processes of genetically defined cell types. We demonstrate SynapTRAP's efficacy and report local translation in the cortex of mice, where we identify a subset of mRNAs that are translated in dendrites by neuronal ribosomes. These mRNAs have disproportionately longer lengths, enrichment for FMRP binding and G-quartets, and their genes are under greater evolutionary constraint in humans. In addition, we show that alternative splicing likely regulates this phenomenon. Overall, SynapTRAP allows for rapid isolation of cell-type-specific localized translation and is applicable to classes of previously inaccessible neuronal and non-neuronal cells
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.