ArticleJournal of leukocyte biology2018
Rolling neutrophils form tethers and slings under physiologic conditions in vivo.
Article in Journal of leukocyte biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Monitoring Circulating Myeloid Cells in Peritonitis with an In Vivo Imaging Flow Cytometer.Biomolecules · 2024Article
- Nanoscopic Characterization of Cell Migration under Flow Using Optical and Electron Microscopy.Analytical chemistry · 2023Article
- A humanized βCell reports · 2022Article
- Selectin-Mediated Signaling-Shedding Light on the Regulation of Integrin Activity in Neutrophils.Cells · 2022Review
- Mitofusin-2 regulates leukocyte adhesion and β2 integrin activation.Journal of leukocyte biology · 2022Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Elongated neutrophil-derived structures are blood-borne microparticles formed by rolling neutrophils during sepsis.The Journal of experimental medicine · 2021Article
- Article
- Visualization of integrin molecules by fluorescence imaging and techniques.Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al · 2021Article
- The effects of neutrophil-generated hypochlorous acid and other hypohalous acids on host and pathogens.Cellular and molecular life sciences : CMLS · 2021Review
- Article
- Neutrophil Recruitment: From Model Systems to Tissue-Specific Patterns.Trends in immunology · 2019Review
- Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms.Science (New York, N.Y.) · 2018Article
- Rolling neutrophils form tethers and slings under physiologic conditions in vivo.Journal of leukocyte biology · 2018Article
- More Than Just a Removal Service: Scavenger Receptors in Leukocyte Trafficking.Frontiers in immunology · 2018Review
- Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44.Blood advances · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Human and mouse neutrophils are known to form tethers when rolling on selectins in vitro. Tethers are ∼0.2 μm thin, ∼5-10 μm-long structures behind rolling cells that can swing around to form slings that serve as self-adhesive substrates. Here, we developed a mouse intravital imaging method, where the neutrophil surface is labeled by injecting fluorescently labeled mAb to Ly-6G. Venules in the cremaster muscle of live mice were imaged at a high frame rate using a confocal microscope equipped with a fast resonant scanner. We observed 270 tethers (median length 3.5 μm) and 31 slings (median length 6.9 µm) on 186 neutrophils of 15 mice. Out of 199 tether break events, 123 were followed by immediate acceleration of the rolling cell, which shows that tethers are load-bearing structures in vivo. In venules with a high wall shear stress (WSS; > 12 dyn/cm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.