Evidence map›Paper›PMID 28821281›Full record

ArticleBreast cancer research : BCR2017

White blood cell DNA methylation and risk of breast cancer in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial (PLCO).

Susan R Sturgeon, J Richard Pilsner, Kathleen F Arcaro, Kaoru Ikuma, Haotian Wu, Soon-Mi Kim, Nayha Chopra-Tandon, Adam R Karpf, Regina G Ziegler, Catherine Schairer and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Breast cancer research : BCR, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 3 pooled it
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 3 syntheses or guidelines pooled it, 11 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Susan R SturgeonDepartment of Biostatistics and Epidemiology, University of Massachusetts-Amherst, 715 North Pleasant Street, Arnold House 407, Amherst, MA, 01003, USA. ssturgeon@schoolph.umass.edu.
J Richard PilsnerDepartment of Environmental Health Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Kathleen F ArcaroDepartment of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Kaoru IkumaDepartment of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
Haotian WuDepartment of Environmental Health Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Soon-Mi KimDepartment of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
Nayha Chopra-TandonDepartment of Biostatistics and Epidemiology, University of Massachusetts-Amherst, 715 North Pleasant Street, Arnold House 407, Amherst, MA, 01003, USA.
Adam R KarpfEppley Institute and Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Regina G ZieglerEpidemiology and Biostatistics Program, Division of Cancer Epidemiology and Genetics, National Cancer institute, Bethesda, MD, 20892, USA.
Catherine SchairerEpidemiology and Biostatistics Program, Division of Cancer Epidemiology and Genetics, National Cancer institute, Bethesda, MD, 20892, USA.
Raji BalasubramanianDepartment of Biostatistics and Epidemiology, University of Massachusetts-Amherst, 715 North Pleasant Street, Arnold House 407, Amherst, MA, 01003, USA.
David A ReckhowDepartment of Civil and Environmental Engineering, University of Massachusetts, Amherst, MA, 01003, USA.
University of Massachusetts Amherst · USDivision of Cancer Epidemiology and GeneticsNational Cancer Institute · USUniversity of Nebraska Medical Center · US

Funding

Epigenotyping in Peripheral Blood Cell DNA and Risk of Breast CancerR15CA170111 · NCI · UNIVERSITY OF MASSACHUSETTS AMHERST · PI STURGEON, SUSAN R · 2013 to 2013
$415k
NCI NIH HHS R15 CA170111
6 · The paper itself

Abstract

backgroundSeveral studies have suggested that global DNA methylation in circulating white blood cells (WBC) is associated with breast cancer risk.

methodsTo address conflicting results and concerns that the findings for WBC DNA methylation in some prior studies may reflect disease effects, we evaluated the relationship between global levels of WBC DNA methylation in white blood cells and breast cancer risk in a case-control study nested within the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial (PLCO) cohort. A total of 428 invasive breast cancer cases and 419 controls, frequency matched on age at entry (55-59, 60-64, 65-69, ≥70 years), year of entry (on/before September 30, 1997, on/after October 1, 1997) and period of DNA extraction (previously extracted, newly extracted) were included. The ratio of 5-methyl-2' deoxycytidine [5-mdC] to 2'-deoxyguanine [dG], assuming [dG] = [5-mdC] + [2'-deoxycytidine [dC]] (%5-mdC), was determined by liquid chromatography-electrospray ionization-tandem mass spectrometry, an especially accurate method for assessing total genomic DNA methylation.

resultsOdds ratio (OR) estimates and 95% confidence intervals (CI) for breast cancer risk adjusted for age at entry, year of entry, and period of DNA extraction, were 1.0 (referent), 0.89 (95% CI, 0.6-1.3), 0.88 (95% CI, 0.6-1.3), and 0.84 (95% CI, 0.6-1.2) for women in the highest compared to lowest quartile levels of %5md-C (p for trend = .39). Effects did not meaningfully vary by time elapsed from WBC collection to diagnosis. DISCUSSION: These results do not support the hypothesis that global DNA hypomethylation in WBC DNA is associated with increased breast cancer risk prior to the appearance of clinical disease.

Indexed as

LeukocytesNeoplastic Cells, CirculatingBiomarkers, TumorBreast NeoplasmsBreast Neoplasms, MaleClinical Trials as TopicColorectal NeoplasmsDNA MethylationFemaleHumansLung NeoplasmsMaleOvarian NeoplasmsProstatic NeoplasmsRisk FactorsBiomarkers, Tumor%5mdCBreast cancerCohortGlobal DNA methylationWhite blood cells

Identifiers

PMID28821281
PMCPMC5563066
OpenAlexW2745701492

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.