Evidence map›Paper›PMID 28821247›Full record

ArticleMicrobial cell factories2017

A cost-effective approach to produce

Jesús Nicolás Carcelén, Juan Manuel Marchante-Gayón, Pablo Rodríguez González, Luis Valledor, María Jesús Cañal, José Ignacio García Alonso

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Review
  3. Toxins · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jesús Nicolás CarcelénDepartment of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
Juan Manuel Marchante-GayónDepartment of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
Pablo Rodríguez GonzálezDepartment of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
Luis ValledorDepartment of Organisms and Systems Biology, University of Oviedo, C/Catedrático Rodrigo Uría s/n, 33071, Oviedo, Spain. valledorluis@uniovi.es.ORCID http://orcid.org/0000-0002-0636-365X
María Jesús CañalDepartment of Organisms and Systems Biology, University of Oviedo, C/Catedrático Rodrigo Uría s/n, 33071, Oviedo, Spain.
José Ignacio García AlonsoDepartment of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006, Oviedo, Spain.
Universidad de Oviedo · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of enriched stable isotopes is of outstanding importance in chemical metrology as it allows the application of isotope dilution mass spectrometry (IDMS). Primary methods based on IDMS ensure the quality of the analytical measurements and traceability of the results to the international system of units. However, the synthesis of isotopically labelled molecules from enriched stable isotopes is an expensive and a difficult task. Either chemical and biochemical methods to produce labelled molecules have been proposed, but so far, few cost-effective methods have been described.

resultsThe aim of this study was to use the microalgae Chlamydomonas reinhardtii to produce, at laboratory scale,

conclusionsPreviously reported biochemical methods to produce isotopically labelled proteins have been applied in the fields of proteomics and fluxomics. For these approaches, low amounts of products are required and the isotopic enrichment of the molecules has never been properly determined. So far, only

Indexed as

Algal ProteinsAmino AcidsAmmonium ChlorideChlamydomonas reinhardtiiCulture MediaGas Chromatography-Mass SpectrometryIsotope LabelingNitrogen IsotopesAlgal ProteinsAmino AcidsAmmonium ChlorideCulture MediaNitrogen-15Nitrogen Isotopes15N-labelled amino acidsBiosynthesisChlamydomonas reinhardtiiGC–MSIsotopic enrichmentProtein extraction

Identifiers

PMID28821247
PMCPMC5563056
OpenAlexW2748424082

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.