ArticleInternational journal of molecular sciences2017
High-Throughput Screening Assay for Laccase Engineering toward Lignosulfonate Valorization.
Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 27 citations in OpenAlex.
- Insight into the Thermal Washing Mechanism of Sodium Lignosulfonate Alkyl/Sodium Persulfate Compound on Oily Sludge.International journal of molecular sciences · 2024Article
- Article
- Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes.Bioresources and bioprocessing · 2023Article
- Tailor-made alkaliphilic and thermostable fungal laccases for industrial wood processing.Biotechnology for biofuels and bioproducts · 2022Article
- A comparative study of greener alternatives for nanocellulose production from sugarcane bagasse.Bioresources and bioprocessing · 2021Article
- Fractionated Lignosulfonates for Laccase-Catalyzed Oxygen-Scavenging Films and Coatings.Molecules (Basel, Switzerland) · 2021Article
- Characterization of a Dye-Decolorizing Peroxidase fromJournal of fungi (Basel, Switzerland) · 2021Article
- A highly stable laccase obtained by swapping the second cupredoxin domain.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are the main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation of lignosulfonates with fungal oxidoreductases, such as laccases, can increase the molecular weight of lignosulfonates by the cross-linking of lignin phenols. To advance in this direction, we describe here the development of a high-throughput screening (HTS) assay for the directed evolution of laccases, with lignosulfonate as substrate and the Folin-Ciocalteau reagent (FCR), to detect the decrease in phenolic content produced upon polymerization of lignosulfonate by the enzyme. Once the reaction conditions were adjusted to the 96-well-plate format, the enzyme for validating the assay was selected from a battery of high-redox-potential laccase variants functionally expressed in
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