ArticleScientific reports2017
Soft electroporation for delivering molecules into tightly adherent mammalian cells through 3D hollow nanoelectrodes.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Solid-State Nanopores for Spatially Resolved Chemical Neuromodulation.Nano letters · 2024Article
- Nanoinjection: A Platform for Innovation in Ex Vivo Cell Engineering.Accounts of chemical research · 2024Article
- Using Human-Induced Pluripotent Stem Cell Derived Neurons on Microelectrode Arrays to Model Neurological Disease: A Review.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Electroactive nanoinjection platform for intracellular delivery and gene silencing.Journal of nanobiotechnology · 2023Article
- Recent Advances in Microscale Electroporation.Chemical reviews · 2022Review
- Semi-Implantable Bioelectronics.Nano-micro letters · 2022Review
- Cellular nanointerface of vertical nanostructure arrays and its applications.Nanoscale advances · 2022Review
- Advances in Cell-Conductive Polymer Biointerfaces and Role of the Plasma Membrane.Chemical reviews · 2022Article
- Microfluidic mechanoporation for cellular delivery and analysis.Materials today. Bio · 2022Review
- Tutorial: using nanoneedles for intracellular delivery.Nature protocols · 2021Review
- In situ electroporation of mammalian cells through SiOScientific reports · 2021Article
- Nanoneedle-Based Materials for Intracellular Studies.Advances in experimental medicine and biology · 2021Article
- High Throughput and Highly Controllable Methods for In Vitro Intracellular Delivery.Small (Weinheim an der Bergstrasse, Germany) · 2020Review
- Impedimetric Detection and Electromediated Apoptosis of Vascular Smooth Muscle Using Microfabricated Biosensors for Diagnosis and Therapeutic Intervention in Cardiovascular Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Microfluidic Multielectrode Arrays for Spatially Localized Drug Delivery and Electrical Recordings of Primary Neuronal Cultures.Frontiers in bioengineering and biotechnology · 2020Article
- Opportunities and dilemmas ofRSC advances · 2019Review
- Nanostructured Materials for Intracellular Cargo Delivery.Accounts of chemical research · 2019Review
- Nanostraw membrane stamping for direct delivery of molecules into adhesive cells.Scientific reports · 2019Article
- On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes.Nano letters · 2019Article
- Enhanced Raman Investigation of Cell Membrane and Intracellular Compounds by 3D Plasmonic Nanoelectrode Arrays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation of in-vitro cultured cells is widely used in biological and medical areas to deliver molecules of interest inside cells. Since very high electric fields are required to electroporate the plasma membrane, depending on the geometry of the electrodes the required voltages can be very high and often critical to cell viability. Furthermore, in traditional electroporation configuration based on planar electrodes there is no a priori certain feedback about which cell has been targeted and delivered and the addition of fluorophores may be needed to gain this information. In this study we present a nanofabricated platform able to perform intracellular delivery of membrane-impermeable molecules by opening transient nanopores into the lipid membrane of adherent cells with high spatial precision and with the application of low voltages (1.5-2 V). This result is obtained by exploiting the tight seal that the cells present with 3D fluidic hollow gold-coated nanostructures that act as nanochannels and nanoelectrodes at the same time. The final soft-electroporation platform provides an accessible approach for controlled and selective drug delivery on ordered arrangements of cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.