ArticleScientific reports2017
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Optical coherence photoacoustic microscopy for 3D cancer model imaging with AI-assisted organoid analysis.Light, science & applications · 2026Article
- Recent advances in oblique plane microscopy.Nanophotonics (Berlin, Germany) · 2023Review
- Mesoscopic oblique plane microscopy with a diffractive light-sheet for large-scale 4D cellular resolution imaging.Optica · 2022Article
- Wide field-of-view volumetric imaging by a mesoscopic scanning oblique plane microscopy with switchable objective lenses.Quantitative imaging in medicine and surgery · 2021Article
- Is oblique scanning laser ophthalmoscope applicable to human ocular optics? A feasibility study using an eye model for volumetric imaging.Journal of biophotonics · 2020Article
- Real-time volumetric microscopy of in vivo dynamics and large-scale samples with SCAPE 2.0.Nature methods · 2019Article
- Volumetric fluorescein angiography (vFA) by oblique scanning laser ophthalmoscopy in mouse retina at 200 B-scans per second.Biomedical optics express · 2019Article
- Volumetric fluorescence retinal imagingBiomedical optics express · 2018Article
- Visible-light optical coherence tomography: a review.Journal of biomedical optics · 2017Review
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Authors and funding
5 authors.
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Abstract
Multi-modal three dimensional (3D) optical imaging combining both structural sensitivity and molecular specificity is highly desirable in biomedical research. In this paper, we present a method termed oblique scanning laser microscopy (OSLM) to combine optical coherence tomography (OCT), for simultaneously volumetric structural and molecular imaging with cellular resolution in all three dimensions. Conventional 3D laser scanning fluorescence microscopy requires repeated optical sectioning to create z-stacks in depth. Here, the use of an obliquely scanning laser eliminates the z-stacking process, then allows highly efficient 3D OCT and fluorescence imaging by using only one raster scan. The current setup provides ~3.6 × 4.2 × 6.5 μm resolution in fluorescence imaging, ~7 × 7 × 3.5 μm in OCT in three dimensions, and the current speed of imaging is up to 100 frames per second (fps) over a volume about 0.8 × 1 × 0.5 mm
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Registered trials
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