ArticleScientific reports2017
The saliva microbiome profiles are minimally affected by collection method or DNA extraction protocols.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03641989 (Correlation Between Oral Health and Systemic Inflammation), which is not on this map. Cited by 85 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Correlation Between Oral Health and Systemic Inflammation (COHESION)
Who cites it
85 citing papers in PubMed, 2 syntheses or guidelines pooled it, 136 citations in OpenAlex.
- Extensive comparison of salivary collection, transportation, preparation, and storage methods: a systematic review.BMC oral health · 2024Pooled it
- Healthy Patients Are Not the Best Controls for Microbiome-Based Clinical Studies: Example of Sjögren's Syndrome in a Systematic Review.Frontiers in immunology · 2021Pooled it
- Detection of Salivary Small Extracellular Vesicles Associated Inflammatory Cytokines Gene Methylation in Gingivitis.International journal of molecular sciences · 2020Trial
- Chronic fatigue syndrome patients have alterations in their oral microbiome composition and function.PloS one · 2018Trial
- The Mechanism and Pathways of Formation and Modification of Salivary Metabolic Profile in Cancer.Current issues in molecular biology · 2026Review
- Comparison of oral mycobiota diversity among healthy removable acrylic denture wearers and nondenture wearers: An exploratory pilot study.Journal of Indian Prosthodontic Society · 2026Article
- Host depletion kits improve microbiome analyses in environmental samples: seagrass as a test case.ISME communications · 2026Article
- Salivaomics: New Frontiers in Studying the Relationship Between Periodontal Disease and Alzheimer's Disease.Metabolites · 2025Review
- Standardizing the approach to clinical-based human microbiome research: from clinical information collection to microbiome profiling and human resource utilization.Osong public health and research perspectives · 2025Article
- Recent advancements in artificial intelligence-powered cancer prediction from oral microbiome.Periodontology 2000 · 2025Review
- Article
- Saliva assay: a call for methodological standardization.Journal of periodontal & implant science · 2025Review
- Salivary Inflammatory Mediator Profiles in Periodontal and Peri-Implant Health and Disease: A Cross-Sectional Study.Clinical implant dentistry and related research · 2025Article
- Impact of increasedJournal of oral microbiology · 2025Article
- Functional profiling of the oral microbiome reveals microbial and oncogenic signatures in never-smoking female patients with oral squamous cell carcinoma.Journal of oral microbiology · 2025Article
- Smoking Impacts Alzheimer's Disease Progression Through Oral Microbiota Modulation.Molecular neurobiology · 2025Review
- Oral microbiota dysbiosis in pediatric patients undergoing treatment for acute lymphoid leukemia a preliminary study.Genetics and molecular biology · 2025Article
- Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing.NPJ biofilms and microbiomes · 2024Article
- Human Salivary Microbiota Diversity According to Ethnicity, Sex, TRPV1 Variants and Sensitivity to Capsaicin.International journal of molecular sciences · 2024Article
- Impact of cell lysis treatment before saliva metagenomic DNA extraction on the oral microbiome and the associated resistome.Clinical and experimental dental research · 2024Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saliva has attracted attention as a diagnostic fluid due to the association of oral microbiota with systemic diseases. However, the lack of standardised methods for saliva collection has led to the slow uptake of saliva in microbiome research. The aim of this study was to systematically evaluate the potential effects on salivary microbiome profiles using different methods of saliva collection, storage and gDNA extraction. Three types of saliva fractions were collected from healthy individuals with or without the gDNA stabilising buffer. Subsequently, three types of gDNA extraction methods were evaluated to determine the gDNA extraction efficiencies from saliva samples. The purity of total bacterial gDNA was evaluated using the ratio of human β-globin to bacterial 16S rRNA PCR while 16S rRNA gene amplicon sequencing was carried out to identify the bacterial profiles present in these samples. The quantity and quality of extracted gDNA were similar among all three gDNA extraction methods and there were no statistically significant differences in the bacterial profiles among different saliva fractions at the genus-level of taxonomic classification. In conclusion, saliva sampling, processing and gDNA preparation do not have major influence on microbiome profiles.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.