ArticleScientific reports2017
Fast wide-volume functional imaging of engineered in vitro brain tissues.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Evaluating chemical effects on human neural cells through calcium imaging and deep learning.iScience · 2024Article
- Human iPSC-Derived Cortical Neurons Display Homeostatic Plasticity.Life (Basel, Switzerland) · 2022Article
- Review
- Lipopolysaccharide-induced neuroinflammation disrupts functional connectivity and community structure in primary cortical microtissues.Scientific reports · 2021Article
- Spontaneous and Perturbational Complexity in Cortical Cultures.Brain sciences · 2021Article
- A Semiautomatic Multi-Label Color Image Segmentation Coupling Dirichlet Problem and Colour Distances.Journal of imaging · 2021Article
- Inferring Excitatory and Inhibitory Connections in Neuronal Networks.Entropy (Basel, Switzerland) · 2021Article
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Volumetric Lissajous confocal microscopy with tunable spatiotemporal resolution.Biomedical optics express · 2020Article
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Functional Characterization of Three-Dimensional Cortical Cultures for In Vitro Modeling of Brain Networks.iScience · 2020Article
- Retinal and Brain Organoids: Bridging the Gap BetweenFrontiers in neuroscience · 2020Review
- 3D Bioprinted Human Cortical Neural Constructs Derived from Induced Pluripotent Stem Cells.Journal of clinical medicine · 2019Article
- Mechano-sensitization of mammalian neuronal networks through expression of the bacterial large-conductance mechanosensitive ion channel.Journal of cell science · 2018Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The need for in vitro models that mimic the human brain to replace animal testing and allow high-throughput screening has driven scientists to develop new tools that reproduce tissue-like features on a chip. Three-dimensional (3D) in vitro cultures are emerging as an unmatched platform that preserves the complexity of cell-to-cell connections within a tissue, improves cell survival, and boosts neuronal differentiation. In this context, new and flexible imaging approaches are required to monitor the functional states of 3D networks. Herein, we propose an experimental model based on 3D neuronal networks in an alginate hydrogel, a tunable wide-volume imaging approach, and an efficient denoising algorithm to resolve, down to single cell resolution, the 3D activity of hundreds of neurons expressing the calcium sensor GCaMP6s. Furthermore, we implemented a 3D co-culture system mimicking the contiguous interfaces of distinct brain tissues such as the cortical-hippocampal interface. The analysis of the network activity of single and layered neuronal co-cultures revealed cell-type-specific activities and an organization of neuronal subpopulations that changed in the two culture configurations. Overall, our experimental platform represents a simple, powerful and cost-effective platform for developing and monitoring living 3D layered brain tissue on chip structures with high resolution and high throughput.
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