ArticleBiosensors & bioelectronics2018
A single-tube approach for in vitro diagnostics using diatomaceous earth and optical sensor.
Article in Biosensors & bioelectronics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Material-to-Application Integration: Rapid Fabrication of Field-Deployable Hydrogel-SiOAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Amine-Modified Diatomaceous Earth Syringe Platform (DeSEI) for Efficient and Cost-Effective EV Isolation.International journal of molecular sciences · 2025Article
- Immuno-SPR biosensor for the detection of Brucella abortus.Scientific reports · 2023Article
- Self-directed molecular diagnostics (SdMDx) system for COVID-19 via one-pot processing.Sensors and actuators. B, Chemical · 2023Article
- Multicenter Testing of a Simple Molecular Diagnostic System for the Diagnosis of Mycobacterium Tuberculosis.Biosensors · 2023Article
- Dimethyl 3,3'-dithiobispropionimidate-functionalized diatomaceous earth particles for efficient biomolecule separation.Scientific reports · 2020Article
- A single-tube sample preparation method based on a dual-electrostatic interaction strategy for molecular diagnosis of gram-negative bacteria.Mikrochimica acta · 2020Article
- A biocomposite-based rapid sampling assay for circulating cell-free DNA in liquid biopsy samples from human cancers.Scientific reports · 2020Article
- Multi-Sample Preparation Assay for Isolation of Nucleic Acids Using Bio-Silica with Syringe Filters.Micromachines · 2020Article
- A robust, hand-powered, instrument-free sample preparation system for point-of-care pathogen detection.Scientific reports · 2019Article
- A Sample Preparation Technique Using Biocompatible Composites for Biomedical Applications.Molecules (Basel, Switzerland) · 2019Article
- Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA.BioMed research international · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Versatile, simple and efficient sample preparation is desirable for point-of-care testing of emerging diseases such as zoonoses, but current sample preparation assays are insensitive, labour-intensive and time-consuming and require multiple instruments. We developed a single-tube sample preparation approach involving direct pathogen enrichment and extraction from human specimens using diatomaceous earth (DE). Amine-modified DE was used to directly enrich a zoonotic pathogen, Brucella, in a large sample volume. Next, a complex of amine-modified DE and dimethyl suberimidate was used for nucleic acid extraction from the enriched pathogen. Using our single-tube approach, the pathogen can be enriched and extracted within 60min at a level of 1 colony formation unit (CFU) from a 1ml sample volume in the same tube. The performance of this approach is 10-100 times better than that of a commercial kit (10
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