Evidence map›Paper›PMID 28818084›Full record

ArticleMalaria journal2017

Do the venous blood samples replicate malaria parasite densities found in capillary blood? A field study performed in naturally-infected asymptomatic children in Cameroon.

Maurice M Sandeu, Albert N Bayibéki, Majoline T Tchioffo, Luc Abate, Geoffrey Gimonneau, Parfait H Awono-Ambéné, Sandrine E Nsango, Diadier Diallo, Antoine Berry, Gaétan Texier and 1 more

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Article in Malaria journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
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  10. Uptake ofFrontiers in microbiology · 2020
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  13. Review
  14. Observational
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Maurice M SandeuLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon.
Albert N BayibékiLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon.
Majoline T TchioffoLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon.
Luc AbateUMR MIVEGEC, Institut de Recherche pour le Développement, 911 Avenue Agropolis, BP64501, 34394, Montpellier Cedex, France.
Geoffrey GimonneauUMR MIVEGEC, Institut de Recherche pour le Développement, 911 Avenue Agropolis, BP64501, 34394, Montpellier Cedex, France.
Parfait H Awono-AmbénéLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon.
Sandrine E NsangoLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon.
Diadier DialloPATH Malaria Vaccine Initiative, Washington, DC, 20001, USA.
Antoine BerryCentre de Physiopathologie de Toulouse Purpan, INSERM U1043, CNRS, UMR5282, Université de Toulouse III, BP 3028, 31024, Toulouse Cedex 03, France.
Gaétan TexierCentre d'épidémiologie et de santé publique des armées, 111 avenue de la Corse, BP40026, 13568, Marseille Cedex 02, France.
Isabelle MorlaisLaboratoire de Recherche sur le Paludisme, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, BP288, Yaoundé, Cameroon. isabelle.morlais@ird.fr.ORCID 0000-0002-2962-7670

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe measure of new drug- or vaccine-based approaches for malaria control is based on direct membrane feeding assays (DMFAs) where gametocyte-infected blood samples are offered to mosquitoes through an artificial feeder system. Gametocyte donors are identified by the microscopic detection and quantification of malaria blood stages on blood films prepared using either capillary or venous blood. However, parasites are known to sequester in the microvasculature and this phenomenon may alter accurate detection of parasites in blood films. The blood source may then impact the success of mosquito feeding experiments and investigations are needed for the implementation of DMFAs under natural conditions.

methodsThick blood smears were prepared from blood obtained from asymptomatic children attending primary schools in the vicinity of Mfou (Cameroon) over four transmission seasons. Parasite densities were determined microscopically from capillary and venous blood for 137 naturally-infected gametocyte carriers. The effect of the blood source on gametocyte and asexual stage densities was then assessed by fitting cumulative link mixed models (CLMM). DMFAs were performed to compare the infectiousness of gametocytes from the different blood sources to mosquitoes.

resultsPrevalence of Plasmodium falciparum asexual stages among asymptomatic children aged from 4 to 15 years was 51.8% (2116/4087). The overall prevalence of P. falciparum gametocyte carriage was 8.9% and varied from one school to another. No difference in the density of gametocyte and asexual stages was found between capillary and venous blood. Attempts to perform DMFAs with capillary blood failed.

conclusionsPlasmodium falciparum malaria parasite densities do not differ between capillary and venous blood in asymptomatic subjects for both gametocyte and trophozoite stages. This finding suggests that the blood source should not interfere with transmission efficiency in DMFAs.

Indexed as

AdolescentCameroonCapillariesChildChild, PreschoolFemaleHumansMalaria, FalciparumMaleParasitemiaPlasmodium falciparumPrevalenceVeinsBlood sourceCameroonMalariaMembrane feedingPlasmodium falciparum

Identifiers

PMID28818084
PMCPMC5561596

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.