ArticleFEMS microbiology letters2017
Identification of biosynthetic gene clusters from metagenomic libraries using PPTase complementation in a Streptomyces host.
Article in FEMS microbiology letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Establishing conserved biosynthetic gene clusters of the phylum Myxococcota.Applied and environmental microbiology · 2026Article
- Genomic characterization and pathogenic mechanisms ofNew microbes and new infections · 2025Article
- Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial Strain MSr12020.Microorganisms · 2022Article
- Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity.Biology · 2022Review
- Strategies to access biosynthetic novelty in bacterial genomes for drug discovery.Nature reviews. Drug discovery · 2022Review
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Strategies for Natural Products Discovery from Uncultured Microorganisms.Molecules (Basel, Switzerland) · 2021Review
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Genome Features and Secondary Metabolites Biosynthetic Potential of the ClassFrontiers in microbiology · 2019Article
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- A Novel Rapid Fungal Promoter Analysis System Using the Phosphopantetheinyl Transferase Gene,Mycobiology · 2018Article
- Streptomycetes: Surrogate hosts for the genetic manipulation of biosynthetic gene clusters and production of natural products.Biotechnology advancesReview
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The majority of environmental bacteria are not readily cultured in the lab, leaving the natural products they make inaccessible using culture-dependent discovery methods. Cloning and heterologous expression of DNA extracted from environmental samples (environmental DNA, eDNA) provides a means of circumventing this discovery bottleneck. To facilitate the identification of clones containing biosynthetic gene clusters, we developed a model heterologous expression reporter strain Streptomyces albus::bpsA ΔPPTase. This strain carries a 4΄-phosphopantetheinyl transferase (PPTase)-dependent blue pigment synthase A gene, bpsA, in a PPTase deletion background. eDNA clones that express a functional PPTase restore production of the blue pigment, indigoidine. As PPTase genes often occur in biosynthetic gene clusters (BGCs), indigoidine production can be used to identify eDNA clones containing BGCs. We screened a soil eDNA library hosted in S. albus::bpsA ΔPPTase and identified clones containing non-ribosomal peptide synthetase (NRPS), polyketide synthase (PKS) and mixed NRPS/PKS biosynthetic gene clusters. One NRPS gene cluster was shown to confer the production of myxochelin A to S. albus::bpsA ΔPPTase.
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