ArticlePloS one2017
Contractile actomyosin arcs promote the activation of primary mouse T cells in a ligand-dependent manner.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanotransduction through T cell receptors: consensus, controversies and future outlooks.Experimental & molecular medicine · 2026Review
- Regulation of actin cytoskeletal dynamics in T cell development and function.Frontiers in immunology · 2025Review
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Semmaphorin 3 A causes immune suppression by inducing cytoskeletal paralysis in tumour-specific CD8Nature communications · 2024Article
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Nanotopography modulates cytoskeletal organization and dynamics during T cell activation.Molecular biology of the cell · 2022Article
- Forces in stem cells and cancer stem cells.Cells & development · 2022Review
- Article
- Programmable DNA-augmented hydrogels for controlled activation of human lymphocytes.Nanomedicine : nanotechnology, biology, and medicine · 2021Article
- Actomyosin dynamics modulate microtubule deformation and growth during T-cell activation.Molecular biology of the cell · 2021Article
- LFA-1 and kindlin-3 enable the collaborative transport of SLP-76 microclusters by myosin and dynein motors.Journal of cell science · 2021Article
- Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine.Cytoskeleton (Hoboken, N.J.) · 2021Review
- The role of actin and myosin in antigen extraction by B lymphocytes.Seminars in cell & developmental biology · 2020Review
- Review
- Multiple actin networks coordinate mechanotransduction at the immunological synapse.The Journal of cell biology · 2020Article
- Phosphorylation of nephrin induces phase separated domains that move through actomyosin contraction.Molecular biology of the cell · 2019Article
- Mechanosensing through immunoreceptors.Nature immunology · 2019Article
- Origin, Organization, Dynamics, and Function of Actin and Actomyosin Networks at the T Cell Immunological Synapse.Annual review of immunology · 2019Review
- Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Cytoskeletal Control of Antigen-Dependent T Cell Activation.Cell reports · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Mechano-transduction is an emerging but still poorly understood component of T cell activation. Here we investigated the ligand-dependent contribution made by contractile actomyosin arcs populating the peripheral supramolecular activation cluster (pSMAC) region of the immunological synapse (IS) to T cell receptor (TCR) microcluster transport and proximal signaling in primary mouse T cells. Using super resolution microscopy, OT1-CD8+ mouse T cells, and two ovalbumin (OVA) peptides with different affinities for the TCR, we show that the generation of organized actomyosin arcs depends on ligand potency and the ability of myosin 2 to contract actin filaments. While weak ligands induce disorganized actomyosin arcs, strong ligands result in organized actomyosin arcs that correlate well with tension-sensitive CasL phosphorylation and the accumulation of ligands at the IS center. Blocking myosin 2 contractility greatly reduces the difference in the extent of Src and LAT phosphorylation observed between the strong and the weak ligand, arguing that myosin 2-dependent force generation within actin arcs contributes to ligand discrimination. Together, our data are consistent with the idea that actomyosin arcs in the pSMAC region of the IS promote a mechano-chemical feedback mechanism that amplifies the accumulation of critical signaling molecules at the IS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.