ArticlePloS one2017
Dextran sodium sulfate (DSS) induces necrotizing enterocolitis-like lesions in neonatal mice.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 52 citations in OpenAlex.
- IL-10 attenuates metabolic dysfunction-associated steatotic liver disease via modulation of hepatic responses to lipotoxicity.JCI insight · 2026Article
- Murine intestinal epithelial cells and lymphocytes undergo contrasting inflammatory shifts during gastrointestinal development.Pediatric research · 2026Article
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- Multi-strain probiotic administration decreases necrotizing enterocolitis severity and alters the epigenetic profile in mice.Pediatric research · 2025Article
- Reducing dextran sodium sulfate interference with gene expression quantification in a mouse model of colitis.Journal of animal science · 2025Article
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- Iron modulates barrier integrity and stem cell function of small intestine during experimental colitis.Frontiers in nutrition · 2025Article
- Worldwide research tendency and collaborations on models of necrotizing enterocolitis: bibliometric exploration over the past three decades.Annals of medicine and surgery (2012) · 2025Review
- Immunological aspects of necrotizing enterocolitis models: a review.Frontiers in immunology · 2024Review
- Models of necrotizing enterocolitis.Seminars in perinatology · 2023Review
- State-of-the-art review and update ofFrontiers in pediatrics · 2023Review
- CXCL1/CXCR2 is involved in white matter injury in neonatal rats via the gut-brain axis.BMC neuroscience · 2022Article
- Intra-Amniotic Administration-An Emerging Method to Investigate Necrotizing Enterocolitis, In Vivo (Nutrients · 2022Article
- Association of neutropenia at disease onset with severe surgical necrotizing enterocolitis and higher mortality: A retrospective study.Frontiers in surgery · 2022Article
- Neutrophil Extracellular Traps in Colorectal Cancer Progression and Metastasis.International journal of molecular sciences · 2021Review
- Blood group AB increases risk for surgical necrotizing enterocolitis and focal intestinal perforation in preterm infants with very low birth weight.Scientific reports · 2021Article
- Fermented Rice Bran Supplementation Prevents the Development of Intestinal Fibrosis Due to DSS-Induced Inflammation in Mice.Nutrients · 2021Article
- Curcumin Alleviated Dextran Sulfate Sodium-Induced Colitis by Regulating M1/M2 Macrophage Polarization and TLRs Signaling Pathway.Evidence-based complementary and alternative medicine : eCAM · 2021Article
- A Mouse Model of Necrotizing Enterocolitis.Methods in molecular biology (Clifton, N.J.) · 2021Article
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Authors and funding
15 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNecrotizing enterocolitis (NEC) is an inflammatory bowel disease of preterm human newborns with yet unresolved etiology. An established neonatal murine model for NEC employs oral administration of lipopolysaccharides (LPS) combined with hypoxia/hypothermia. In adult mice, feeding dextran sodium sulfate (DSS) represents a well-established model for experimental inflammatory bowel disease. Here we investigated the effect of DSS administration on the neonatal murine intestine in comparison with the established NEC model.
methods3-day-old C57BL/6J mice were either fed formula containing DSS or LPS. LPS treated animals were additionally stressed by hypoxia/hypothermia twice daily. After 72 h, mice were euthanized, their intestinal tissue harvested and analyzed by histology, qRT-PCR and flow cytometry. For comparison, adult C57BL/6J mice were fed with DSS for 8 days and examined likewise. Untreated, age matched animals served as controls.
resultsAdult mice treated with DSS exhibited colonic inflammation with significantly increased Cxcl2 mRNA expression. In contrast, tissue inflammation in neonatal mice treated with DSS or LPS plus hypoxia/hypothermia was present in colon and small intestine as well. Comparative analysis of neonatal mice revealed a significantly increased lesion size and intestinal Cxcl2 mRNA expression after DSS exposure. Whereas LPS administration mainly induced local neutrophil recruitment, DSS treated animals displayed increased monocytes/macrophages infiltration.
conclusionsOur study demonstrates the potential of DSS to induce NEC-like lesions accompanied by a significant humoral and cellular immune response in the small and large intestine of neonatal mice. The new model therefore represents a good alternative to LPS plus hypoxia/hypothermia administration requiring no additional physical stress.
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