ArticleNature protocols2017
Single-cell mechanogenetics using monovalent magnetoplasmonic nanoparticles.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Magnetic DNA Origami Nanorotors.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Magnetically Controlled Reversible Photomagnetic Nanoactuators for Dynamic Contrast Enhancement in Optical Coherence Tomography.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Electromagnetic wireless remote control of mammalian transgene expression.Nature nanotechnology · 2025Article
- A magnetically powered nanomachine with a DNA clutch.Nature nanotechnology · 2024Article
- Magneto-acoustic protein nanostructures for non-invasive imaging of tissue mechanics in vivo.Nature materials · 2024Article
- Tunable Interparticle Connectivity in Gold Nanosphere Assemblies for Efficient Photoacoustic Conversion.Advanced functional materials · 2023Article
- Hydrogel Magnetomechanical Actuator Nanoparticles for Wireless Remote Control of MechanosignalingNano letters · 2023Article
- Advanced theragnostics for the central nervous system (CNS) and neurological disorders using functional inorganic nanomaterials.Advanced drug delivery reviews · 2023Review
- Single-Molecule Imaging of Membrane Proteins on Vascular Endothelial Cells.Journal of lipid and atherosclerosis · 2023Review
- Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling.Nature cell biology · 2022Article
- Valence-controlled protein conjugation on nanoparticlesChemical science · 2022Article
- Biophysical Approaches for Applying and Measuring Biological Forces.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Introducing stimulogenetics, unraveling pertinent semantic ambiguity, and determining clinical relevance among novel neuromodulation strategies.Biology methods & protocols · 2022Article
- Modulating cell signalling in vivo with magnetic nanotransducers.Nature reviews. Methods primers · 2022Article
- Development of Integrated Systems for On-Site Infection Detection.Accounts of chemical research · 2021Review
- Non-contact long-range magnetic stimulation of mechanosensitive ion channels in freely moving animals.Nature materials · 2021Article
- Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.Nature biomedical engineering · 2020Article
- Small, Clickable, and Monovalent Magnetofluorescent Nanoparticles Enable Mechanogenetic Regulation of Receptors in a Crowded Live-Cell Microenvironment.Nano letters · 2019Article
- A magnetic resonance tuning sensor for the MRI detection of biological targets.Nature protocols · 2018Article
- Magnetic Nanotweezers for Interrogating Biological Processes in Space and Time.Accounts of chemical research · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Spatiotemporal interrogation of signal transduction at the single-cell level is necessary to answer a host of important biological questions. This protocol describes a nanotechnology-based single-cell and single-molecule perturbation tool, termed mechanogenetics, that enables precise spatial and mechanical control over genetically encoded cell-surface receptors in live cells. The key components of this tool are a magnetoplasmonic nanoparticle (MPN) actuator that delivers defined spatial and mechanical cues to receptors through target-specific one-to-one engagement and a micromagnetic tweezers (μMT) that remotely controls the magnitude of force exerted on a single MPN. In our approach, a SNAP-tagged cell-surface receptor of interest is conjugated with a single-stranded DNA oligonucleotide, which hybridizes to its complementary oligonucleotide on the MPN. This protocol consists of four major stages: (i) chemical synthesis of MPNs, (ii) conjugation with DNA and purification of monovalent MPNs, (iii) modular targeting of MPNs to cell-surface receptors, and (iv) control of spatial and mechanical properties of targeted mechanosensitive receptors in live cells by adjusting the μMT-to-MPN distance. Using benzylguanine (BG)-functionalized MPNs and model cell lines expressing either SNAP-tagged Notch or vascular endothelial cadherin (VE-cadherin), we provide stepwise instructions for mechanogenetic control of receptor clustering and for mechanical receptor activation. The ability of this method to differentially control spatial and mechanical inputs to targeted receptors makes it particularly useful for interrogating the differential contributions of each individual cue to cell signaling. The entire procedure takes up to 1 week.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.