ArticleNature protocols2017
Meganuclease-assisted generation of stable transgenics in the sea anemone Nematostella vectensis.
Article in Nature protocols, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Evolutionary rewiring of the endomesoderm gene regulatory networks specify skeleton secreting cells in stony corals.bioRxiv : the preprint server for biology · 2026Article
- Establishment of a transgenic protocol inZoological research · 2026Article
- Decoding cnidarian cell type gene regulation.Nature ecology & evolution · 2026Article
- Heat Stress Drives Rapid Viral and Antiviral Innate Immunity Activation in Hexacorallia.Molecular ecology · 2025Article
- Chordin-mediated BMP shuttling patterns the secondary body axis in a cnidarian.Science advances · 2025Article
- The cellular basis of feeding-dependent body size plasticity in sea anemones.Development (Cambridge, England) · 2024Article
- A population of Vasa2 and Piwi1 expressing cells generates germ cells and neurons in a sea anemone.Nature communications · 2024Article
- Doublecortin-like kinase is required for cnidocyte development in Nematostella vectensis.Neural development · 2024Article
- Venom trade-off shapes interspecific interactions, physiology, and reproduction.Science advances · 2024Article
- A noveliScience · 2024Article
- Analysis of SMAD1/5 target genes in a sea anemone reveals ZSWIM4-6 as a novel BMP signaling modulator.eLife · 2024Article
- Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- NvPrdm14d-expressing neural progenitor cells contribute to non-ectodermal neurogenesis in Nematostella vectensis.Nature communications · 2023Article
- An ancestral Wnt-Brachyury feedback loop in axial patterning and recruitment of mesoderm-determining target genes.Nature ecology & evolution · 2022Article
- The architecture and operating mechanism of a cnidarian stinging organelle.Nature communications · 2022Article
- Article
- Functional Characterization of the Cnidarian Antiviral Immune Response Reveals Ancestral Complexity.Molecular biology and evolution · 2021Article
- Comparison of the Feasibility, Efficiency, and Safety of Genome Editing Technologies.International journal of molecular sciences · 2021Review
- Review
- Toxin-like neuropeptides in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The sea anemone Nematostella vectensis is a model system used by a rapidly growing research community for comparative genomics, developmental biology and ecology. Here, we describe a microinjection procedure for creating stable transgenic lines in Nematostella based on meganuclease (I-SceI)-assisted integration of a transgenic cassette into the genome. The procedure describes the preparation of the reagents, microinjection of the transgenesis vector and the husbandry of transgenic animals. The microinjection setup differs from those of previously published protocols by the use of a holding capillary mounted on an inverted fluorescence microscope. In one session of injections, a single researcher can microinject up to 1,300 zygotes with a reporter construct digested with the meganuclease I-SceI. Under optimal conditions, fully transgenic heterozygous F1 animals can be obtained within 4-5 months of the injections, with a germ-line transmission efficiency of ∼3%. The method is versatile and, after a short training phase, can be carried out by any researcher with basic training in molecular biology. Flexibility of construct design enables this method to be used for numerous applications, including the functional dissection of cis-regulatory elements, subcellular localization of proteins, detection of protein-binding partners, ectopic expression of genes of interest, lineage tracing and cell-type-specific reporter gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.