ArticleRNA biology2017
Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa.
Article in RNA biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 97 citations in OpenAlex.
- Paternal cytokine administration alters sperm small ncRNAs and offspring brain and behavior.Molecular psychiatry · 2026Article
- SUN5 forms a regular protein lattice reinforcing the sperm head-tail junction.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Epididymosomes: Composition and Functions for Sperm Maturation.Advances in anatomy, embryology, and cell biology · 2026Review
- A father's legacy: the sperm epigenome, preimplantation development, and paternal environment.Epigenomics · 2025Review
- Phthalate exposure to adult mice alters non-coding RNAs in cauda epididymosomes.Environmental pollution (Barking, Essex : 1987) · 2025Article
- Role of Seminal Exosomes in Reproduction.Journal of cellular physiology · 2025Review
- The composition of human sperm sncRNAome: a cross-country small RNA profiling.Reproductive biology and endocrinology : RB&E · 2025Article
- Sperm-borne small non-coding RNAs: potential functions and mechanisms as epigenetic carriers.Cell & bioscience · 2025Review
- Subchronic elevation in ambient temperature drives alterations to the sperm epigenome and accelerates early embryonic development in mice.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Episodic ozone exposure in Long-Evans rats has limited effects on cauda sperm motility and non-coding RNA populations.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Future in the past: paternal reprogramming of offspring phenotype and the epigenetic mechanisms.Archives of toxicology · 2024Review
- Single-cell and spatial transcriptomic investigation reveals the spatiotemporal specificity of the beta-defensin gene family during mouse sperm maturation.Cell communication and signaling : CCS · 2024Article
- ONC201 in Combination with Paxalisib for the Treatment of H3K27-Altered Diffuse Midline Glioma.Cancer research · 2023Article
- Glucocorticoid exposure modifies the miRNA profile of sperm in the guinea pig: Implications for intergenerational transmission.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- Parental Preconception and Pre-Hatch Exposure to a Developmental Insult Alters Offspring's Gene Expression and Epigenetic Regulations: An Avian Model.International journal of molecular sciences · 2023Article
- HA-tag CD63 is a novel conditional transgenic approach to track extracellular vesicle interactions with sperm and their transfer at conception.Scientific reports · 2023Article
- New horizons in human sperm selection for assisted reproduction.Frontiers in endocrinology · 2023Review
- sncRNAs in Epididymosomes: The Contribution to Embryonic Development and Offspring Health.International journal of molecular sciences · 2022Review
- 3D structure and in situ arrangements of CatSper channel in the sperm flagellum.Nature communications · 2022Article
- Paternal environmental exposure-induced spermatozoal small noncoding RNA alteration meditates the intergenerational epigenetic inheritance of multiple diseases.Frontiers of medicine · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition of which is markedly influenced by their epididymal transit. Thus, although microRNAs (miRNAs) are highly represented in the spermatozoa of the proximal epididymis, this sRNA class is largely diminished in mature spermatozoa of the distal epididymis. Coincident with this, a substantial enrichment in Piwi-interacting RNA (piRNA) abundance in cauda spermatozoa was detected. Further, features of cauda piRNAs, including; predominantly 29-31 nts in length; preference for uracil at their 5' terminus; no adenine enrichment at piRNA nt 10, and; predominantly mapping to intergenic regions of the mouse genome, indicate that these piRNAs are generated by the PIWIL1-directed primary piRNA production pathway. Accordingly, PIWIL1 was detected via immunoblotting and mass spectrometry in epididymal spermatozoa. These data provide insight into the complexity and dynamic nature of the sRNA profile of spermatozoa and raise the intriguing prospect that piRNAs are generated in situ in maturing spermatozoa. Such information is of particular interest in view of the potential role for paternal sRNAs in influencing conception, embryo development and intergenerational inheritance.
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Registered trials
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