ArticleScientific reports2017
Functional characterization of PBP1 gene in Helicoverpa armigera (Lepidoptera: Noctuidae) by using the CRISPR/Cas9 system.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 70 citations in OpenAlex.
- Pheromone-Binding Proteins in Pest Control: From Molecular Insights to Real-World Applications.Journal of agricultural and food chemistry · 2025Review
- Loss-of-function in testis-specific serine/threonine protein kinase triggers male infertility in an invasive moth.Communications biology · 2024Article
- Mannitol mediates the mummification behavior ofFrontiers in microbiology · 2024Article
- CRISPR/Cas9-Mediated genomic knock out of tyrosine hydroxylase and yellow genes in cricket Gryllus bimaculatus.PloS one · 2023Article
- Molecular Characterization and Expression Patterns of Two Pheromone-Binding Proteins from the Diurnal MothInternational journal of molecular sciences · 2022Article
- Review
- Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera.BMC biology · 2022Article
- Pheromone binding protein is involved in temporal olfactory resolution in the silkmoth.iScience · 2021Article
- The role of SNMPs in insect olfaction.Cell and tissue research · 2021Review
- Identification of Candidate Olfactory Genes inFrontiers in physiology · 2021Article
- Molecular mechanisms of olfactory detection in insects: beyond receptors.Open biology · 2020Article
- Identification and Expression Profiles of 14 Odorant-Binding Protein Genes From Pieris rapae (Lepidoptera: Pieridae).Journal of insect science (Online) · 2020Article
- Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of theFrontiers in physiology · 2020Article
- Odor-Specific Deactivation Defects in aGenetics · 2019Article
- CRISPR/Cas9-mediated PBP1 and PBP3 mutagenesis induced significant reduction in electrophysiological response to sex pheromones in male Chilo suppressalis.Insect science · 2019Article
- CRISPR/Cas9 Genome Editing Introduction and Optimization in the Non-model InsectFrontiers in physiology · 2019Article
- In vivo functional characterisation of pheromone binding protein-1 in the silkmoth, Bombyx mori.Scientific reports · 2018Article
- The Odorant Binding Protein 6 Expressed in Sensilla Chaetica Displays Preferential Binding Affinity to Host Plants Volatiles inFrontiers in physiology · 2018Article
- Identification of Chemosensory Genes Based on the Transcriptomic Analysis of Six Different Chemosensory Organs inFrontiers in physiology · 2018Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pheromone binding proteins (PBPs) are thought to play crucial roles in perception of the sex pheromones particularly in noctuid moths, but this is rarely in vivo evidenced due to lacking an effective technique. Here, we reported an in vivo functional study of PBP1 in the important lepidopteran pest Helicoverpa armigera (HarmPBP1), by using the CRISPR/Cas9 system. Efficient and heritable mutagenesis was achieved by egg injection of mixture of Cas9-mRNA and HarmPBP1-sgRNA. The TA cloning and sequencing revealed various insertion and/or deletion (indel) mutations at the target site. Among those, one mutation resulted in a premature stop codon at the target site, which led to a highly truncated protein with only 10 amino acids. The HarmPBP1 with this mutation would completely loss its function, and thus was used to select the homozygous mutant insects for functional analysis. The electroantennogram recording showed that the mutant male adults displayed severely impaired responses to all three sex pheromone components (Z11-16:Ald, Z9-16:Ald and Z9-14:Ald). Our study provides the first in vivo evidence that HarmPBP1 plays important role in perception of female sex pheromones, and also an effective methodology for using CRISPR/Cas9 system in functional genetic study in H. armigera as well as other insects.
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