ArticlePloS one2017
Durum wheat miRNAs in response to nitrogen starvation at the grain filling stage.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- MicroRNA and target gene dynamics in potato under nitrogen deficiency.Scientific reports · 2025Article
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- Plant miRNAs influence soil bacterial growth and amino acid uptake, restructuring community composition.ISME communications · 2025Article
- miRNA mediated regulation of nitrogen response and nitrogen use efficiency of plants: the case of wheat.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Current Progress and Future Prospect of Wheat Genetics Research towards an Enhanced Nitrogen Use Efficiency.Plants (Basel, Switzerland) · 2023Review
- A Survey of the Transcriptomic Resources in Durum Wheat: Stress Responses, Data Integration and Exploitation.Plants (Basel, Switzerland) · 2023Review
- Functional role of microRNA in the regulation of biotic and abiotic stress in agronomic plants.Frontiers in genetics · 2023Review
- Nitrogen use efficiency-a key to enhance crop productivity under a changing climate.Frontiers in plant science · 2023Review
- Nitrate/ammonium-responsive microRNA-mRNA regulatory networks affect root system architecture in Populus × canescens.BMC plant biology · 2022Article
- Integrated analysis of miRNAome transcriptome and degradome reveals miRNA-target modules governing floral florescence development and senescence across early- and late-flowering genotypes in tree peony.Frontiers in plant science · 2022Article
- Identification and Functional Prediction of Poplar Root circRNAs Involved in Treatment With Different Forms of Nitrogen.Frontiers in plant science · 2022Article
- R2R3-MYBs in Durum Wheat: Genome-Wide Identification, Poaceae-Specific Clusters, Expression, and Regulatory Dynamics Under Abiotic Stresses.Frontiers in plant science · 2022Article
- Noncoding-RNA-Mediated Regulation in Response to Macronutrient Stress in Plants.International journal of molecular sciences · 2021Review
- Small RNA, Transcriptome and Degradome Analysis of the Transgenerational Heat Stress Response Network in Durum Wheat.International journal of molecular sciences · 2021Article
- Comparative Analysis Based on Transcriptomics and Metabolomics Data Reveal Differences between Emmer and Durum Wheat in Response to Nitrogen Starvation.International journal of molecular sciences · 2021Article
- Nitrogen Starvation-Responsive MicroRNAs Are Affected by Transgenerational Stress in Durum Wheat Seedlings.Plants (Basel, Switzerland) · 2021Article
- Small RNAs and their targets are associated with the transgenerational effects of water-deficit stress in durum wheat.Scientific reports · 2021Article
- Signaling Responses to N Starvation: Focusing on Wheat and Filling the Putative Gaps With Findings Obtained in Other Plants. A Review.Frontiers in plant science · 2021Review
- Biochemical and Genetic Approaches Improving Nitrogen Use Efficiency in Cereal Crops: A Review.Frontiers in plant science · 2021Review
- Roles of Non-Coding RNAs in Response to Nitrogen Availability in Plants.International journal of molecular sciences · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Durum wheat highly depends on nitrogen for seed development and yield, and the obtainment of varieties with a better nitrogen use efficiency is crucial to reduce production costs and environmental pollution. In this study, sequencing of two small RNA libraries obtained from tissues of Ciccio and Svevo cultivars grown under nitrogen starvation conditions produced 84 novel, and 161 conserved miRNAs. Of these, 7 novel and 13 known miRNAs were newly identified in this work. Quantitative PCR analysis of selected miRNAs highlighted that the expression levels of some of them depends on the tissue and on the cultivar, Svevo being the most responsive to nitrogen starvation. A number of target genes were predicted to be involved in nitrogen metabolism. An inverse correlation for the qPCR expression data of miRNA/target pairs miR399b/PHO2, miR393c/AFB2, ttu-novel-61/CCAAT-TF was observed in specific tissues or cultivar. Especially, ttu-novel-61 was down-regulated and its target CCAAT-TF up-regulated in almost all tissues both in Svevo and in Ciccio. Moreover, CCAAT-TF was confirmed to be cleaved by ttu-novel-61 at the expected site. The discovery of miRNAs involved in the response to nitrogen stress represents an important step towards functional analyses, with the final aim to design strategies for improving nitrogen use efficiency in durum wheat.
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