ArticleNature ecology & evolution2017
Experimental test and refutation of a classic case of molecular adaptation in Drosophila melanogaster.
Article in Nature ecology & evolution, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Epistasis among clustered lineage-specific amino acid substitutions in the Drosophila Trio protein.PLoS genetics · 2026Article
- Deep homology and design of proteasome chaperone proteins in Candidozyma auris.Nature communications · 2026Article
- From Natural Behavior to Drug Screening: Invertebrates as Models to Study Mechanisms Associated with Alcohol Use Disorders.Current topics in behavioral neurosciences · 2025Review
- Heat tolerance, oxidative stress response tuning and robust gene activation in early-stageProceedings. Biological sciences · 2024Article
- Functional Divergence of the bag-of-marbles Gene in the Drosophila melanogaster Species Group.Molecular biology and evolution · 2022Article
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- The curious consistency of carbon biosignatures over billions of years of Earth-life coevolution.The ISME journal · 2021Review
- The genomics of rapid climatic adaptation and parallel evolution in North American house mice.PLoS genetics · 2021Article
- Different genetic basis for alcohol dehydrogenase activity and plasticity in a novel alcohol environment for Drosophila melanogaster.Heredity · 2020Article
- Ancestral-sequence reconstruction unveils the structural basis of function in mammalian FMOs.Nature structural & molecular biology · 2020Article
- Article
- Fitness effects but no temperature-mediated balancing selection at the polymorphicProceedings of the National Academy of Sciences of the United States of America · 2019Article
- A clinal polymorphism in the insulin signaling transcription factor foxo contributes to life-history adaptation in Drosophila.Evolution; international journal of organic evolution · 2019Article
- A major role for noncoding regulatory mutations in the evolution of enzyme activity.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Population-specific dynamics and selection patterns of transposable element insertions in European natural populations.Molecular ecology · 2019Article
- Functional Analysis of a Putative Target of Spatially Varying Selection in theG3 (Bethesda, Md.) · 2019Article
- Ancient mechanisms for the evolution of the bicoid homeodomain's function in fly development.eLife · 2018Article
- Multinucleotide mutations cause false inferences of lineage-specific positive selection.Nature ecology & evolution · 2018Article
- Reconstructing Ancient Proteins to Understand the Causes of Structure and Function.Annual review of biophysics · 2017Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Identifying the genetic basis for adaptive differences between species requires explicit tests of historical hypotheses concerning the effects of past changes in gene sequence on molecular function, organismal phenotype and fitness. We address this challenge by combining ancestral protein reconstruction with biochemical experiments and physiological analysis of transgenic animals that carry ancestral genes. We tested a widely held hypothesis of molecular adaptation-that changes in the alcohol dehydrogenase protein (ADH) along the lineage leading to Drosophila melanogaster increased the catalytic activity of the enzyme and thereby contributed to the ethanol tolerance and adaptation of the species to its ethanol-rich ecological niche. Our experiments strongly refute the predictions of the adaptive ADH hypothesis and caution against accepting intuitively appealing accounts of historical molecular adaptation that are based on correlative evidence. The experimental strategy we employed can be used to decisively test other adaptive hypotheses and the claims they entail about past biological causality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.