ArticleScientific reports2017
Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Modulating Chromophore Flexibility in GEVIs through Threonine-Based Molecular Switches Reveals an Influence of Membrane Curvature on Protein Activity.ACS sensors · 2025Article
- From Plankton to Primates: How VSP Sequence Diversity Shapes Voltage Sensing.International journal of molecular sciences · 2025Article
- Plasma membrane-endoplasmic reticulum coupling probed with genetically-encoded voltage sensors.bioRxiv : the preprint server for biology · 2025Article
- Mapping Synaptic Activity at the Population and Cellular Levels with Genetically Encoded Voltage Indicators (GEVIs).Methods in molecular biology (Clifton, N.J.) · 2025Article
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Review
- Exploiting the molecular diversity of the synapse to investigate neuronal communication: A guide through the current toolkit.The European journal of neuroscience · 2022Review
- A trafficking motif alters GEVI activity implicating persistent protein interactions at the membrane.Biophysical reports · 2022Article
- Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size ofFrontiers in cell and developmental biology · 2022Article
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires.Biophysical journal · 2021Article
- Improving the flexibility of genetically encoded voltage indicators via intermolecular FRET.Biophysical journal · 2021Article
- Large-Scale 3D Two-Photon Imaging of Molecularly Identified CA1 Interneuron Dynamics in Behaving Mice.Neuron · 2020Article
- Article
- Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1.iScience · 2020Article
- Optical voltage imaging in neurons: moving from technology development to practical tool.Nature reviews. Neuroscience · 2019Review
- Genetic voltage indicators.BMC biology · 2019Review
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Red Fluorescent Genetically Encoded Voltage Indicators with Millisecond Responsiveness.Sensors (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
An improved genetically encoded voltage indicator (GEVI) was achieved by altering the charge composition of the region linking the voltage-sensing domain of the GEVI to a pH-sensitive fluorescent protein. Negatively charged linker segments reduced the voltage-dependent optical signal while positively charged linkers increased the signal size. Arginine scanning mutagenesis of the linker region improved the signal size of the GEVI, Bongwoori, yielding fluorescent signals as high as 20% ΔF/F during the firing of action potentials. The speed of this new sensor was also capable of optically resolving action potentials firing at 65 Hz. This large signal size enabled individual pixels to become surrogate electrodes. Plotting the highest correlated pixels based only on fluorescence changes reproduced the image of the neuron exhibiting activity. Furthermore, the use of a pH-sensitive fluorescent protein facilitated the detection of the acidification of the neuron during the firing of action potentials.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.