ArticleScientific reports2017
Ligand binding modes from low resolution GPCR models and mutagenesis: chicken bitter taste receptor as a test-case.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Integrated Metabolomics and Transcriptomics Reveal Bitter Compounds and Synthetic Pathways in the Special-Germplasm Bitter-TastingPlants (Basel, Switzerland) · 2026Article
- Functional Decline of a Bitter Receptor Gene in New World Vultures.Molecular biology and evolution · 2025Article
- Exploring TAS2R46 biomechanics through molecular dynamics and network analysis.Frontiers in molecular biosciences · 2024Article
- Bitter Taste Perception in Chickens.The journal of poultry science · 2022Article
- Fragment-based in silico design of SARS-CoV-2 main protease inhibitors.Chemical biology & drug design · 2021Article
- Bitter Taste and Olfactory Receptors: Beyond Chemical Sensing in the Tongue and the Nose.The Journal of membrane biology · 2021Article
- In Silico Molecular Study of Tryptophan Bitterness.Molecules (Basel, Switzerland) · 2020Article
- Structure-Function Analyses of Human Bitter Taste Receptors-Where Do We Stand?Molecules (Basel, Switzerland) · 2020Review
- Rational design of agonists for bitter taste receptor TAS2R14: from modeling to bench and back.Cellular and molecular life sciences : CMLS · 2020Article
- Responsiveness Expressions of Bitter Taste Receptors Against Denatonium Benzoate and Genistein in the Heart, Spleen, Lung, Kidney, and Bursa Fabricius of Chinese Fast Yellow Chicken.Animals : an open access journal from MDPI · 2019Article
- Beyond the Flavour: The Potential Druggability of Chemosensory G Protein-Coupled Receptors.International journal of molecular sciences · 2019Review
- BitterDB: taste ligands and receptors database in 2019.Nucleic acids research · 2019Article
- Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors inBioMed research international · 2019Article
- Understanding Ligand Binding to G-Protein Coupled Receptors Using Multiscale Simulations.Frontiers in molecular biosciences · 2019Review
- Independent Evolution of Strychnine Recognition by Bitter Taste Receptor Subtypes.Frontiers in molecular biosciences · 2018Article
- Molecular Features Underlying Selectivity in Chicken Bitter Taste Receptors.Frontiers in molecular biosciences · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bitter taste is one of the basic taste modalities, warning against consuming potential poisons. Bitter compounds activate members of the bitter taste receptor (Tas2r) subfamily of G protein-coupled receptors (GPCRs). The number of functional Tas2rs is species-dependent. Chickens represent an intriguing minimalistic model, because they detect the bitter taste of structurally different molecules with merely three bitter taste receptor subtypes. We investigated the binding modes of several known agonists of a representative chicken bitter taste receptor, ggTas2r1. Because of low sequence similarity between ggTas2r1 and crystallized GPCRs (~10% identity, ~30% similarity at most), the combination of computational approaches with site-directed mutagenesis was used to characterize the agonist-bound conformation of ggTas2r1 binding site between TMs 3, 5, 6 and 7. We found that the ligand interactions with N93 in TM3 and/or N247 in TM5, combined with hydrophobic contacts, are typically involved in agonist recognition. Next, the ggTas2r1 structural model was successfully used to identify three quinine analogues (epiquinidine, ethylhydrocupreine, quinidine) as new ggTas2r1 agonists. The integrated approach validated here may be applicable to additional cases where the sequence identity of the GPCR of interest and the existing experimental structures is low.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.